天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (4): 639-646.doi: 10.16333/j.1001-6880.2022.4.012

• 开发研究 • 上一篇    下一篇

发酵葛根与水提葛根的抗氧化活性与延缓线虫衰老的作用研究

赵   丹，吴   迪，李   萌*，王昌涛，张佳婵，王冬冬   

  1. 北京工商大学化学与材料工程学院 北京市植物资源研究与开发重点实验室，北京 100048 

  • 出版日期:2022-04-29 发布日期:2022-04-29
  • 基金资助:
    国家自然科学基金（31971382）

Study on the antioxidant activity of fermented and aqueous-extracted Pueraria lobata and their effect on delaying Caenorhabditis elegans aging

ZHAO Dan,WU Di,LI Meng*,WANG Chang-tao,ZHANG Jia-chan,WANG Dong-dong   

  1. Beijing Key Laboratory of Plant Resources Research and Development,College of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China 
  • Online:2022-04-29 Published:2022-04-29

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摘要： 为挖掘葛根在抗氧化和抗衰老领域中的应用，使用发酵法和水提法对葛根活性物质进行提取，通过生化方法对其体外抗氧化功效进行检测，并以线虫为模式生物对两种提取物的抗衰老功效进行了比较研究。结果表明6.8%体积浓度的葛根发酵液的总抗氧化能力、清除羟自由基和超氧阴离子的能力均高于葛根水提液。与对照组相比，葛根发酵液能够显著延长线虫寿命（P<0.05），同时促进线虫产卵量增加4.8%，葛根发酵液作用后线虫耐热时间增加至对照组的111.6%。基因检测显示葛根发酵液能够抑制促衰老基因daf-2的表达，上调延缓衰老基因daf-16的表达，并对耐热基因hsp-16.2、抗氧化基因sod-3表达均有促进作用，因此相比对照组，葛根发酵液通过提高线虫的抗氧化性和对温度的耐受性来延长线虫寿命。葛根发酵液良好的抗氧化效果以及抗衰老的功效为其在相关食品、化妆品领域的应用提供参考。

关键词: 葛根, 发酵, 抗氧化, 线虫, 衰老

Abstract:

To explore the application of Pueraria lobata in antioxidant and anti-aging field,two methods of fermentation and aqueous extraction were used to extract the active substances of P.lobata.The biochemical experiments were detected to evaluate the antioxidant activity of the extracts and Caenorhabditis elegans were used as model animal to detect the anti-aging effect.Results showed that 6.8% fermented broth of P. lobata (FBP) had better antioxidant ability than aqueous extract of P. lobata (AEP).The antioxidant ability included the total antioxidant capacity,the scavenging capacity of hydroxyl free radicals and superoxide anions.Compared with control group,FBP could significantly prolong the lifespan of C .elegans (P<0.05),and promote the fecundity of C. elegans by 4.8%.At the same time,the heat-resistant time of C. elegans was increased to 111.6% of the control group.Real-time quantitative PCR results showed that FBP could inhibit the expression of the pro-aging gene daf-2 and up-regulate the expression of the anti-aging gene daf-16.In addition,the FBP promote the expression of the downstream heat-resistant genehsp-16.2 and the antioxidant gene of sod-3.In conclusion,the FBP could prolong the lifespan of C. elegans by improving the antioxidant activity and heat resistance of the C. elegans.The good antioxidant and anti-aging effect of FBP provide a reference for its application in the field of anti-aging related food and cosmetics.

Key words: Pueraria lobata, fermentation, antioxidant, Caenorhabditis elegans, aging

中图分类号:  TS201.4

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