天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (10): 1707-1712.doi: 10.16333/j.1001-6880.2022.10.008

• 研究简报 • 上一篇    下一篇

钩藤内生真菌Talaromyces sp.次级代谢产物研究

曾慧婷，叶   琳，易维婷，陈嘉怡，徐珊珊，赵丽娟，曾   禧，袁   涛*   

  1. 江西师范大学生命科学学院，南昌 330022
  • 出版日期:2022-10-28 发布日期:2022-10-31
  • 基金资助:
    江西省“双千计划”（jxsq2018101008）

Study on secondary metabolites of endophytic fungus Talaromyces sp. from Uncaria rhynchophylla

ZENG Hui-ting,YE Lin,YI Wei-ting,CHEN Jia-yi,XU Shan-shan,ZHAO Li-juan,ZENG Xi,YUAN Tao*   

  1. College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China
  • Online:2022-10-28 Published:2022-10-31

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摘要： 综合利用多种色谱分离技术从钩藤内生真菌Talaromyces sp.1.88E的发酵产物中分离得到8个化合物，采用HR-ESI-MS、NMR、IR等波谱解析技术将其鉴定为(Z)-6-methoxy-6-oxo-2-propylhex-2-enoic acid（1）、alteric acid（2）、5′-methoxy-6-methyl-biphenyl-3,4,3′-triol（3）、3-O-methylfunicone（4）、alternariol 1′-hydroxy-9-methyl ether（5）、格链孢酚（6）、5-甲氧基格链孢酚（7）、altenuisol（8）。化合物1为新化合物，化合物2的核磁数据为首次报道。对化合物1~8采用脂多糖（LPS）诱导小鼠单核巨噬细胞RAW 264.7生成NO炎症模型测试抗炎活性，均未表现出抗炎活性。对化合物1~8进行α-葡萄糖苷酶抑制活性测试，化合物2、6对α-葡萄糖苷酶表现出弱的抑制效果，其IC50分别为338.5、375.8 μmol/L。

关键词: 钩藤, 内生真菌, 次级代谢产物, 抗炎活性, α-葡萄糖苷酶

Abstract:

Eight compounds were isolated from the extract of endophytic fungus Talaromyces sp.1.88E from Uncaria rhynchophylla (Miq.) Miq.ex Havil.by various chromatographic techniques.The compounds were identified as (Z)-6-methoxy-6-oxo-2-propylhex-2-enoic acid (1),alteric acid (2),5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (3),3-O-methylfunicone (4),alternariol 1′-hydroxy-9-methyl ether (5),alternariol (6),alternariol methyl ether (7),altenuisol (8) by HR-ESI-MS,NMR,IR and other spectroscopic analysis techniques.Among them,compound 1 was a new fatty acid and NMR data of compound 2 was reported for the first time.Compounds 1-8 showed no obvious inhibition of LPS-induced NO production by RAW 264.7 for the anti-inflammatory test.All isolated compounds were evaluated for the inhibitory activities against α-glucosidase and compounds 2,6 showed weak inhibitory activity with IC50 values of 338.5,and 375.8 μmol/L,respectively.

Key words: Uncaria rhynchophylla, endophytic fungus, secondary metabolites, anti-inflammatory, α-glucosidase

中图分类号:  R284.1

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