In this study,the quality of Imperatae Rhizoma standard decoction from different habitats was evaluated by UPLC fingerprint and quantitative analysis of multiple components.Similarity calculation,cluster analysis (HCA),principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the fingerprint data.The results showed that there were 9 common fingerprint peaks in the fingerprints of 18 batches of Imperatae Rhizoma standard decoction.The similarity of fingerprints ranged from 0.920 to 0.997.HCA divided 18 batches of samples into three categories.The samples from Hebei Province were of their own kind.The samples from Jiangsu Province were crossed with those from Anhui and Henan provinces respectively.PCA showed that,except Jiangsu Province,the differences of common peaks in the fingerprints of Imperatae Rhizoma standard decoction in the same production area were small.OPLS-DA screened three differential marker chromatographic peaks,which were peak 9 (p-hydroxycinnamic acid),peak 6 and peak 5 (chlorogenic acid) respectively.The contents of chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid and p-hydroxycinnamic acid in Imperatae Rhizoma standard decoction were determined.The results showed that the total content of four phenolic acids in Imperatae Rhizoma standard decoction were:Hebei > Anhui > Jiangsu > Henan.The contents of four phenolic acids in samples from different production areas were significantly different.The contents of chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid in the samples from Henan production area were significantly lower than that from other production areas.The average content of p-hydroxycinnamic acid in the samples from Anhui production area was the highest and relatively stable.The total amount of p-hydroxycinnamic acid and four phenolic acids in the samples of Jiangsu production area fluctuated greatly.The established analytical method is stable,reliable and reproducible,which can provide reference for the quality evaluation of Imperatae Rhizoma standard decoction and its related preparations.