The purpose of this study was to explore the anti- nutritional characteristics of Shengxuebao mixture drug residue (SXBR) and its effect on the growth performance of New Zealand rabbits,so as to provide a basis for its feed development.Firstly,the contents of anti-nutritional factors such as lignin,xylan,pectin,tannin and phytic acid in the SXBR,the single traditional Chinese medicines in Shengxuebao mixture and common feed materials were determined.Then,different proportions of SXBR were added to the diet of New Zealand rabbits to determine their nutritional level.After continuous feeding for 30 days,the apparent digestibility of various nutrients and other production performance indicators of New Zealand rabbits were measured.Finally,the UPLC-TOF-MS technology was used to detect the toxic components of SXBR into the blood,and the pathological sections of liver and ileum in each group were observed.The results showed that the lignin content of SXBR (9.64%) was higher than most common feed materials,second only to rice bran.The lignin in SXBR mainly comed from the hard medicinal materials such as Cibotii Rhizoma,Ligustri Lucidi Fructus and Polygoni Multiflori Radix Praeparata.With the addition of SXBR,the nutritional level of feed was gradually improved.The results of in vivo experiments showed that SXBR had no effect on the weight growth of New Zealand rabbits,but when 20% SXBR was added to the diet of rabbits,the apparent digestibility of crude protein decreased significantly (P=0.003).No toxic substances were detected in SXBR serum samples,and there was no toxic effect on liver and intestine.To sum up,the SXBR as a feed additive has good safety,but its lignin level is relatively prominent,which has a great negative impact on the apparent digestibility of crude protein of New Zealand rabbits.Therefore,reducing the lignin content and increasing digestibility of feed protein can be taken as the main direction to solve this problem.