天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (12): 2050-2055.doi: 10.16333/j.1001-6880.2022.12.008

• 研究简报 • 上一篇    下一篇

粉防己根的化学成分及抗肝纤维化活性研究

许进业1，许凤清1,2，孔垂皓1，赵宏苏1,2，王仁中1,2*，吴德玲1,2*   

  1. 1安徽中医药大学药学院；2中药研究与开发安徽省重点实验室，合肥 230012
  • 出版日期:2022-12-28 发布日期:2022-12-29
  • 基金资助:
    安徽省高等学校自然科学研究项目（KJ2021A0594）；中药研究与开发安徽省重点实验室开放基金（AKLPDCM202003）；安徽中医药大学人才支持计划（2020rcyb006）

Chemical constituents from the roots of Stephania tetrandra and their anti-liver fibrosis activity

XU Jin-ye1,XU Feng-qing1,2,KONG Chui-hao1,ZHAO Hong-su1,2,WANG Ren-zhong1,2*,WU De-ling1,2*   

  1. 1School of Pharmacy,Anhui University of Chinese Medicine;2Anhui Province Key Laboratory of Research & Development of Chinese Medicine,Hefei 230012,China
  • Online:2022-12-28 Published:2022-12-29

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摘要：

研究粉防己根的化学成分和抗肝纤维化活性。采用硅胶、Sephadex LH-20、ODS和半制备HPLC对粉防己根的乙醇提取物进行分离纯化得到13个化合物。根据化合物的理化性质和波谱数据鉴定其结构，分别为cyclanoline（1）、coclaurine（2）、5-hydroxymethyl-1-［2-(4-hydroxyphenyl)-ethyl］-1H-pyrrole-2-carbaldehyde（3）、isosalsoline（4）、thalifoline（5）、northalifoline（6）、2-甲基-3-羟基吡啶（7）、(+)-lyoniresinol（8）、icariside B5（9）、丁香酸（10）、对羟基苯乙酸（11）、对羟基苯甲酸（12）、对羟基苯乙酸甲酯（13）。化合物1~13为首次从该植物中分离得到，在千金藤属植物中化合物3、4、6~9也未见报道。初步评价了化合物1~13的抗肝纤维化活性，化合物2、5、6、10~12对TGF-β1诱导的LX-2细胞增殖具有显著抑制作用。

关键词: 粉防己, 化学成分, 分离鉴定, 肝星状细胞

Abstract:

The chemical constituents from roots of Stephania tetrandra and their anti-liver fibrosis activity were studied.The roots of Stephania tetrandra were extracted with EtOH,and the extractions were separated on column chromatography of silica gel,Sephadex LH-20,ODS and semi-preparative HPLC to yield 13 compounds.According to the physical and chemical properties and spectral data,13 compounds were identified as cyclanoline (1),coclaurine (2),5-hydroxymethyl-1-[2-(4-hydroxyphenyl)-ethyl]-1H-pyrrole-2-carbaldehyde (3),isosalsoline (4),thalifoline (5),northalifoline (6),2-methyl-3-hydrox ypyridine (7), (+)-lyoniresinol (8),icariside B5 (9),syringic acid (10),4-hydroxyphenylacetic acid (11),4-hydroxybenzoic acid (12),p-hydroxyphenylacetic acid methyl ester (13).Compounds 1-13 are isolated from this plant for the first time.And compounds 3,4,6-9 have never been reported before from the genus Stephania. The compounds 1-13 were preliminarily examined for anti-liver fibrosis activity.Compounds 2,5,6,10-12 showed antiproliferative activities in LX-2 induced by TGF-β1.

Key words: Stephania tetrandra, chemical constituents, isolation and identification, hepatic stellate cells

中图分类号:  R932

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