HPLC fingerprints of L. chinensis were established,and the contents of diosmin and linarin were determined to provide reference for the quality evaluation of L. chinensis in different production areas.The HPLC fingerprints were established by Thermo Acclaim C₁₈(4.6 mm × 250 mm,5 μm) chromatographic column.The mobile phase was acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution.The flow rate was 1.0 mL per minute with gradient elution.Detection wavelength was 340 nm.The column temperature was 35 ℃.The injection volume was 10 μL.The common peaks were identified by mass spectrometric analysis and comparison with reference substances.With the help of the similarity evaluation system of chromatographic fingerprints of traditional Chinese medicine(2012.130723),the similarity evaluation was carried out on the fingerprints.Cluster analysis(HCA),principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) were used to compare the quality differences of L. chinensis from different habitats.The contents of diosmin and linarin in L. chinensis were determined.The results showed that there were 11 common peaks in the fingerprints of 18 batches of L. chinensis.Four common peaks were confirmed by mass spectrometry analysis and comparison with the reference substance,namely luteolin 7-diglucoside,clerodendrin,diosmin and linarin.Compared with the mzCloud standard database,The structure of the other four common peaks were predicted.The results of similarity evaluation showed that the medicinal materials of L. chinensis in Henan and Anhui provinces were similar,and the medicinal materials of L. chinensis in the other four regions were similar.HCA and PCA divided L. chinensis from different production areas into two categories.The samples from Henan and Anhui provinces were classified into one category,and the samples from Hubei,Guizhou,Jiangxi and Hunan provinces were classified into the other category.The results were basically consistent with the similarity evaluation results.OPLS-DA found 7 differential markers.According to the VIP values,they were peak 2> peak 1> peak 10> peak 11> peak 3> peak 8> peak 5.The content determination results showed that the contents of diosmin and linarin in three batches of L. chinensis from Henan and Anhui provinces were significantly higher than those from other provinces.This method can effectively evaluate the quality difference ofL. chinensis from different producing areas,and provide reference for its quality control and purchase of medicinal materials.