天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (1): 40-46.doi: 10.16333/j.1001-6880.2023.1.005

• 研究论文 • 上一篇    下一篇

千层塔中脂肪族类化学成分的抗肿瘤活性研究

曹   朵1,2，李佳慧3，侯梦阳4，王   燕5，李   涛5*   

  1. 1延安大学生命科学院；2延安大学红枣重点实验室，延安 716000；3安徽理工大学医学院，淮南 232000；4大连理工大学生物工程学院，大连 116000；5上海市普陀区利群医院，上海 200333
  • 出版日期:2023-01-28 发布日期:2023-02-20
  • 基金资助:
    陕西省科学技术协会青年人才托举计划（20220321）;延安大学疫情防控应急科研项目（ydfk067）上海市普陀区卫生健康系统科技创新项目（ptkwws202118）

Anti-tumor activity of aliphatic compounds from Huperzia serrata

CAO Duo1,2, LI Jia-hui3,HOU Meng-yang4,WANG Yan5,LI Tao5*   

  1. 1School of Life Sciences,Yan′an University;2Key Laboratory of Jujube,Yan′an University,Yan′an 716000,China;3School of Medicine,Anhui University of Science and Technology,Huainan 232000,China;4School of Bioengineering,Dalian University of Technology,Dalian 116000,China;5Liqun Hospital,Putuo District,Shanghai,Shanghai 200333,China
  • Online:2023-01-28 Published:2023-02-20

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摘要：

千层塔中脂肪族类化学成分及其抗肿瘤活性研究。在肿瘤抑制活性引导下，采用AB-8大孔树脂、氧化铝、硅胶、Sephadex LH 20和半制备液相等方法，对千层塔甲醇提取物进行较系统分离纯化，并结合现代波谱手段对分离到的化合物进行结构鉴定。共分离鉴定了9个单体化合物，分别为pentyl-4-oxoicosa-2(Z),6(Z)-dienoate（1）、2-(2-ethoxy-1-hydroxy-2-oxoethyl)-2-hydroxy-4-methylpentanoic acid（2）、ethyl (9Z,12Z)-octadeca-9,12-dienoate（3）、(Z)-nonadec-10-enal（4）、油酸酰胺（5）、反式-9-十八碳烯酸（6）、bacillamidin A（7）、(3Z,6Z,9Z)-3,6,9-二十二碳三烯（8）、oplopandiol（9）。其中，化合物1和化合物2为新化合物，化合物3~9系首次从千层塔中分离。肿瘤抑制活性结果显示，化合物1和3对HepG2细胞具有抑制作用，其IC50分别为41.12、80.54 μmol/L。

关键词: 千层塔, 脂肪族类化学成分, 抗肿瘤

Abstract:

The anti-tumor activity of aliphatic in Huperzia serrata was studied. Guided by antitumor activity,the AB-8 macroporous resin,alumina,silica gel,Sephadex LH 20 and pre-HPLC were used to separate and purify the methanol extract of H. serrata.The structures were identified by modern spectroscopic methods.Nine compounds were isolated and identified,which were classified as pentyl-4-oxoicosa-2(Z),6 (Z)-dienoate (1),2-(2-ethoxy-1-hydroxy-2-oxoethyl)-2-hydroxy-4-methylpentanoic acid (2)，ethyl (9Z,12Z)-octadeca-9,12-dienoate (3),(Z)-nonadec-10-enal (4),oleamide (5),trans-octadec-9-enoic acid (6),bacillamidin A (7),(3Z,6Z,9Z)-3,6,9-docosatriene (8),oplopandiol (9).Compounds 1 and 2 were new compounds. Compounds 3-9 were isolated from H. serrata for the first time. The results of HepG2 cells inhibitory activity showed that compounds 1 and 3 had inhibitory effect on HepG2 with IC50 of 41.12 and 80.54 μmol/L, respectively.

Key words: Huperzia serrata, aliphatic compounds, anti-tumor activity

中图分类号:  R284.1 R932

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