Plant seeds are rich in microbial resources.For digging into the endophytic bacteria of seeds of Coptis chinensis,endophytic bacterial strains were isolated from seeds with plate isolation,and the obtained strains were studied on phosphate-dissolving,nitrogen fixation,siderophores and IAA production.The strains with growth-promoting properties were screened,and were tested for growth-promoting effects in combination with the seed germination experiment,as well as molecularly identified.The results showed that seeds of C. chinensis were rich in culturable endophytic bacteria.A total of 57 endophytic bacteria strains were isolated,and 29 strains were identified by 16S rRNA,mainly distributed in 13 genera such as Bacillus,Stenotrophomonas,Microbacterium and Achromobacter.The dominant bacteria were Bacillus (seven strains) and Stenotrophomonas (seven strains),accounting for 24.14% of the total identified strains respectively.The study of growth-promoting characteristics showed that there were two strains with phosphorus-dissolving function,nine strains with nitrogen-fixing function,two siderophores-producing strains,and six strains with IAA-producing function,and their IAA secretion amount ranged from 64.52-203.57 mg/L.Among them,one strain was capable of both phosphate-dissolving and IAA production,and one strain as capable of phosphate-dissolving and siderophore production,and one strain was capable of siderophore production and IAA production.Priestia megaterium and A. spanius could significantly increase the germination percentage of seeds of C. chinensis.This study will provide a theoretical basis for revealing the growth promotion mechanism of seed endophytes and understanding the mechanism of after-ripening and germination of recalcitrant seeds,and also lay a foundation for the development of microbial fertilizer.