基于生物信息学筛选的胰腺癌差异基因及免疫浸润机制预测靶向中药

doi:10.16333/j.1001-6880.2023.2.016

天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (2): 319-331.doi: 10.16333/j.1001-6880.2023.2.016

基于生物信息学筛选的胰腺癌差异基因及免疫浸润机制预测靶向中药

龙思丹1,2，季双双2，陈美池1,2，贠张君2，马银杰1，薛鹏1，朱世杰1*

1中国中医科学院望京医院肿瘤科，北京 100102；2北京中医药大学研究生院，北京 100029

出版日期:2023-02-28 发布日期:2023-03-02
基金资助:
中国中医科学院科技创新工程（CI2021B009）；中国中医科学院望京医院院级课（WJYY2020-02）

Bioinformatics-based screening of pancreatic cancer differential genes and immune infiltration mechanisms to predict targeting of traditional Chinese medicine

LONG Si-dan1,2,JI Shuang-shuang2,CHEN Mei-chi1,2,YUN Zhang-jun2,MA Yin-jie1,XUE Peng1,ZHU Shi-jie1*

1Department of Oncology,Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China;2Graduate School,Beijing University of Chinese Medicine,Beijing 100029,China

Online:2023-02-28 Published:2023-03-02

摘要/Abstract

摘要： 本研究首先通过生物信息学方法得到胰腺癌差异基因2 653个，进一步筛选得到COL1A2等10个核心差异基因，涉及蛋白质消化与吸收等信号通路。然后生存分析表明核心差异基因对胰腺癌患者的无复发生存率具有显著影响，进一步免疫细胞浸润矩阵分析发现胰腺癌组织中CD4⁺记忆T细胞、M2巨噬细胞、树突状细胞等浸润程度高，而CD8⁺T细胞浸润程度低，不同免疫细胞的分布之间存在一定相关性。最后，本研究预测的有效中药多属于清热、补益和祛风湿类，归肝经，其中葶苈子、姜黄等中药与核心基因和相关免疫途径最为密切。

关键词: 胰腺癌, 生物信息学, 免疫浸润, 生存分析, 中药预测

Abstract:

In this study,we first obtained 2 653 differential genes of pancreatic cancer by bioinformatics.Further screening yielded 10 core differential genes,including COL1A2,involved in signaling pathways such as protein digestion and absorption.Survival analysis revealed that core difference genes had a significant effect on recurrence-free survival in pancreatic cancer patients.Immune cell infiltration matrix analysis in pancreatic cancer tissues revealed high levels of CD4⁺ memory T cells,M2 macrophages,and dendritic cells,but low levels of CD8⁺ T cells,with some correlation between the distribution of various immune cells.Finally,the majority of the effective herbal remedies identified in this study were associated with the liver meridians and were classified as clearing heat,tonifying,and dispelling wind-dampness.Among them,Curcumae Longae Rhizoma and Descurainiae Semen Lepidii Semen had the strongest connections to the immune pathways and key genes.

Key words: pancreatic cancer, bioinformatics, immune infiltration, survival analysis, herbal prediction

中图分类号: R285

龙思丹, 季双双, 陈美池, 贠张君, 马银杰, 薛鹏, 朱世杰. 基于生物信息学筛选的胰腺癌差异基因及免疫浸润机制预测靶向中药[J]. 天然产物研究与开发, 2023, 35(2): 319-331.

LONG Si-dan, JI Shuang-shuang, CHEN Mei-chi, YUN Zhang-jun, MA Yin-jie, XUE Peng, ZHU Shi-jie. Bioinformatics-based screening of pancreatic cancer differential genes and immune infiltration mechanisms to predict targeting of traditional Chinese medicine[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(2): 319-331.

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基于生物信息学筛选的胰腺癌差异基因及免疫浸润机制预测靶向中药

Bioinformatics-based screening of pancreatic cancer differential genes and immune infiltration mechanisms to predict targeting of traditional Chinese medicine

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