天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (3): 405-409.doi: 10.16333/j.1001-6880.2023.3.006

• 研究简报 • 上一篇    下一篇

杜仲皮中木脂素类化学成分及其对高糖诱导的肾小球系膜细胞保护作用研究

黄   玲1，苏彦红2，蒋跃平3,4，张   燕1，罗荣耀1，张颖帆5，黄   琪3,4*   

  1. 1大理大学药学院，大理 671000；2山东省沂南县湖头镇卫生院，沂南 276300；3中南大学湘雅医院药学部；4中南大学湘雅医院国家老年疾病临床医学研究中心；5中南大学湘雅医院手术室，长沙 410008
  • 出版日期:2023-03-28 发布日期:2023-03-28
  • 基金资助:
    国家自然科学基金（82003929）；湖南省自然科学基金（2021JJ40993）

 Lignans from Eucommia ulmoides barks and their protective effect on high-glucose-stimulated human renal mesangial cells

HUANG Ling1,SU Yan-hong2,JIANG Yue-ping3,4,ZHANG Yan1,LUO Rong-yao1,ZHANG Ying-fan5,HUANG Qi3,4*   

  1. 1College of Pharmacy,Dali University,Dali 671000,China; 2 Health Center of Hutou Town,Yinan County,Shandong Province,Yinan 276300,China; 3 Department of Pharmacy,Xiangya Hospital,Central South University;4 National Clinical Research Center for Geriatric Disorders,Xiangya Hospital,Central South University;5Operating Room,Xiangya Hospital,Central South University,Changsha 410008,China
  • Online:2023-03-28 Published:2023-03-28

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摘要：

研究杜仲皮中的木脂素类化学成分，并评价其对高糖诱导的人肾小球系膜细胞的保护作用。将杜仲树皮粉碎后，纯化水提取，采用HPD-100大孔吸附树脂、硅胶、Sephadex LH-20、半制备高效液相色谱等方法进行分离，根据理化性质和波谱学方法鉴定化合物结构，从杜仲皮水提物中分离鉴定了6个木脂素类化合物，包括caruilignan D（1）、（-）-表松脂素（2）、（+）-松脂素4-O-β-D-吡喃葡萄糖苷（3）、（-）-松脂素4-O-β-D-吡喃葡萄糖苷（4）、（+）-中脂素4-O-β-D-吡喃葡萄糖苷（5）和（+）-中脂素4′-O-β-D-吡喃葡萄糖苷（6），化合物1为首次从杜仲中分离得到。在高糖诱导异常增殖的肾小球系膜细胞模型中，采用MTT法检测木脂素类化合物干预后的细胞活力。化合物5能剂量依赖性地抑制细胞增殖，对高糖诱导的肾小球系膜细胞具有保护活性。

关键词: 杜仲皮, 化学成分, 木脂素, 结构鉴定, 细胞活力

Abstract:

To study the lignans compound from Eucommia ulmoides barks and evaluate their protective effects on high-glucose-stimulated human renal mesangial cells.The barks of E. ulmoides was crushed and extracted with purified water.HPD-100 macroporous resin,Silica gel,Sephadex LH-20 and Semi-preparative HPLC were used to separate compounds in the aqueous extract,and their structures were identified based on physicochemical properties and spectral analysis.Six lignans were isolated and identified as caruilignan D (1),(-)-epipinoresinol (2),(+)-pinoresinol 4-O-β-D-glucopyranoside (3),(-)-pinoresinol 4-O-β-D-glucopyranoside (4),(+)-medioresinol 4-O-β-D-glucopyranoside (5) and (+)-medioresinol 4′-O-β-D-glucopyranoside (6).Compound 1 was isolated from E. ulmoides for the first time.The effect of isolated lignans on cell viability in high-glucose-stimulated human renal mesangial cells (HRMCs) with abnormal proliferation was evaluated by MTT assay.It was found that compound 5 can inhibit cell proliferation in a dose dependent manner,indicating that compound 5 exerts protective activity on high-glucose-stimulated HRMCs.

Key words: Eucommia ulmoides barks, chemical constituents, lignans, structure identification, cell viability

中图分类号:  R284.1 R285.5

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