天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (3): 410-419.doi: 10.16333/j.1001-6880.2023.3.007

• 研究简报 • 上一篇    下一篇

不同产地紫堇属植物生物碱类成分的研究

桂正杰1，申   凯2，黄明浩1，李光伟1，黄泰奇1，金卫华1*   

  1. 1武汉东湖学院生命科学与化学学院，武汉 430212；2苏州凯祥生物科技有限公司，苏州 215600
  • 出版日期:2023-03-28 发布日期:2023-03-28
  • 基金资助:
    武汉东湖学院“东湖未来之星”大学生科研项目（2022）

Alkaloid constituents in Corydalis from different habitats

GUI Zheng-jie1,SHEN Kai 2, HUANG Ming-hao1,LI Guang-wei 1,HUANG Tai-qi 1,JIN Wei-hua1*   

  1. 1College of Life Science & Chemistry,Wuhan Donghu University,Wuhan 430212,China; 2Catch Bio-science & Technology Co.,Ltd.,Suzhou 215600,China
  • Online:2023-03-28 Published:2023-03-28

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摘要：

为了比较不同产地紫堇属植物的生物碱类成分差异，并探究地锦苗中的生物碱类成分，本文采用高效液相色谱法（HPLC）、酸性染料比色法比较不同产地紫堇属植物的原阿片碱和总生物碱含量差异，并采用超高效液相色谱串联四级杆飞行时间质谱法（UPLC-Q-TOF-MS）分析鉴定地锦苗中生物碱类成分。结果显示，不同产地紫堇属植物的原阿片碱和总生物碱含量差异不明显；通过比对相关文献和质谱数据，确定了UPLC-Q-TOF-MS检测结果中的13种生物碱，包括1个缩醛类丽春花定碱类、1个简单异喹啉类、2个普罗托品类、2个原小檗碱类、1个苯酞异喹啉类、4个苯菲啶类、1个四氢小檗碱类、1个阿朴啡类。上述检测结果可对紫堇属药用植物资源开发提供理论依据。

关键词: 紫堇属, 总生物碱, HPLC, UPLC-Q-TOF-MS

Abstract:

To compare the alkaloid constituents of Corydalis from different habitats and explore the alkaloid constituents in Corydalis sheareri S.Moore.,the HPLC and acid dye colorimetry were used to compare the contents of protopine and total alkaloids in Corydalis from different habitats.Alkaloid constituents in Corydalis sheareri S.Moore. were identified by ultra-performance liquid chromatography-qua-drupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS).The results showed that the contents of protopine and total alkaloids in Corydalis from different habitats were not significantly different.A total of 13 alkaloids were identified by UPLC-Q-TOF-MS through high-resolution mass spectrometry data and relevant literature,including one rhoeadine-type alkaloid,one simple isoquinoline-type alkaloid,two protopine-type alkaloids,two protoberberine-type alkaloids,one phthalide-type alkaloid,four benzo-phenanthridine-type alkaloids,one tetrahydroproto-berberine-type alkaloid,one aporphine-type alkaloid. The results can provide a theoretical datum for the development of medicinal plant resources of Corydalis.

Key words: Corydalis, total alkaloids, HPLC, UPLC-Q-TOF-MS

中图分类号:  R284

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