臭牡丹苯乙醇苷类成分的鉴定及体外抗人肺腺癌A549细胞的机制研究

doi:10.16333/j.1001-6880.2023.3.010

天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (3): 444-452.doi: 10.16333/j.1001-6880.2023.3.010

臭牡丹苯乙醇苷类成分的鉴定及体外抗人肺腺癌A549细胞的机制研究

王芬1，唐皓2，周厚元1，余湘蓉1，朱克俭2，李顺祥1，余娜1*，唐林3*

1湖南中医药大学，长沙 410208；2湖南省中医药研究院附属医院，长沙 410006；3湖南中医药大学第一附属医院，长沙 410007

出版日期:2023-03-28 发布日期:2023-03-28
基金资助:
湖南省自然科学基金（2020JJ5428）；湖南省中医药管理局一般项目（201935）；中药现代化研究室，湖南中医药大学中药学一流学科项目（校行科字［2018］3号）

Identification of phenylethanoid glycosides from Clerodendrum bungei and its mechanism of anti-human lung adenocarcinoma A549 cell in vitro

WANG Fen1,TANG Hao2,ZHOU Hou-yuan1,YU Xiang-rong1,ZHU Ke-jian2,LI Shun-xiang1,YU Na1 *,TANG Lin3*

1School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;2Hunan Academy of Chinese Medicine,Changsha 410006,China;3The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China

Online:2023-03-28 Published:2023-03-28

摘要/Abstract

摘要：

臭牡丹提取物具有良好的抗肿瘤的作用，为了鉴定臭牡丹提取物的成分及探究其对A549细胞体外抑制的影响，采用超高效液相色谱-质谱联用技术、CCK-8、细胞划痕法、Transwell、Western blot、免疫荧光及流式细胞术等方法进行检测。鉴定了臭牡丹提取物中34个苯乙醇苷类化合物，其中类叶升麻苷含量为32.95%，异类叶升麻苷5.49%。体外实验表明，臭牡丹提取物能抑制A549细胞的增殖、迁移和侵袭，且IC₅₀为0.125 mg/mL，还能下调A549细胞中EphA2、p-AKT、mTOR、p-mTOR、p-GSK-3β和β-catenin的表达，上调EphrinA1的表达，减少EphA2在A549细胞膜与膜质上的表达，并将A549细胞周期阻滞于G1/S期。本研究证实臭牡丹地上部分富含苯乙醇苷类成分，尤以类叶升麻苷和异类叶升麻苷为多，且可通过调控EphA2/Akt/mTOR通路达到抗肺癌作用。

关键词: 臭牡丹, 苯乙醇苷, 肺腺癌细胞, EphA2/AKT/mTOR通路

Abstract:

Clerodendrum bungei extract has a good anti-tumor effect.In order to identify the ingredients of the C. bungei extract and explore its impact on the A549 in vitro,the ultra-high-efficiency liquid chromatography-mass spectrometry technology,CCK-8,cell scratch,Transwell,Western blot,immunofluorescence and flow cytometry methods were used to detect. The 34 phenylethanoid glycosides compounds of the C. bungei extract were identified,of which the content of acteoside was 32.95%,and isoacteoside was 5.49%.The C. bungei extract can inhibit the proliferation,migration and invasion of A549 cellsin vitro,and the IC₅₀ is 0.125 mg/mL.In addition,the C. bungei extract can reduce the expression of EphA2,P-AKT,mTOR,P-mTOR,P-GSK-3β,and β-cateninin in A549 cells,increase the expression of EphrinA1,reducing the expression of EphA2 in A549 cell membrane and membrane quality,and blocke the A549 cell cycle to the G1/S stage.This study has confirmed that the C. bungei is rich in phenylethanoid glycosides,especially acteoside and isoacteoside,and can achieve anti-lung cancer effect by regulating the EphA2/AKT/ mTOR pathway.

Key words: Clerodendrum bungei, phenylethanoid glycosides, A549, EphA2/AKT/mTOR pathway

中图分类号: R965.2

王芬, 唐皓, 周厚元, 余湘蓉, 朱克俭, 李顺祥, 余娜, 唐林. 臭牡丹苯乙醇苷类成分的鉴定及体外抗人肺腺癌A549细胞的机制研究[J]. 天然产物研究与开发, 2023, 35(3): 444-452.

WANG Fen, TANG Hao, ZHOU Hou-yuan, YU Xiang-rong, ZHU Ke-jian, LI Shun-xiang, YU Na , TANG Lin . Identification of phenylethanoid glycosides from Clerodendrum bungei and its mechanism of anti-human lung adenocarcinoma A549 cell in vitro[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(3): 444-452.

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臭牡丹苯乙醇苷类成分的鉴定及体外抗人肺腺癌A549细胞的机制研究

Identification of phenylethanoid glycosides from Clerodendrum bungei and its mechanism of anti-human lung adenocarcinoma A549 cell in vitro

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