天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (4): 602-606.doi: 10.16333/j.1001-6880.2023.4.006

• 研究简报 • 上一篇    下一篇

芫花花蕾中一个新的瑞香烷二萜类化合物

杨春霞1,关吉利1,张   蕾2*   

  1. 1三亚市中医院,三亚 572000;2海南医学院第一附属医院,海口 570102
  • 出版日期:2023-04-24 发布日期:2023-04-24
  • 基金资助:
    海南省卫生计生行业科研项目(16A200077)

A new daphnane diterpenoid from the flower buds of Daphne genkwa

YANG Chun-xia1,GUAN Ji-li1,ZHANG Lei2 *   

  1. 1Sanya Traditional Chinese Medicine Hospital,Sanya 572000,China;2The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China
  • Online:2023-04-24 Published:2023-04-24

摘要: 为了研究中药芫花(Daphne genkwa)的干燥花蕾化学成分及其抑制皮肤病原真菌活性,采用正相硅胶及半制备HPLC等多种色谱技术对芫花花蕾95%乙醇提取物进行分离纯化得到2个瑞香烷型二萜类化合物,并通过MS、NMR和ECD等波谱技术和文献数据比对鉴定化合物结构,分别为daphgenin A(1)和yuanhuakine B(2),其中化合物1为新的瑞香烷型二萜类化合物。同时,结合MIC法测定该化合物对皮肤真菌犬小孢子菌、红色毛癣菌、须癣毛癣菌和马拉色菌的抑制活性,结果表明化合物12对皮肤病原真菌有一定的抑制活性,化合物1对红色毛癣菌和须癣毛癣菌的抑制活性与阳性对照组相当(MIC值4.0 μg/mL),化合物2对红色毛癣菌显示显著的抑制活性,其MIC值为2.0 μg/mL。

关键词: 芫花, 化学成分, 瑞香烷型二萜, 抗皮肤真菌活性

Abstract:

To study the chemical constituents and their anti-dermatophyte activities of Daphne genkwa.Two daphnane diterpenoid derivatives were isolated from the 95% ethanol extract of Daphne genkwa using various column chromatography,such as silica gel and preparative HPLC.And the structures were identified by NMR,MS,and ECD spectroscopic techniques,as well as comparison with literatures.Compound 1 was a new daphnane diterpenoid compound,and named as daphgenin A (1),compound 2 was identified as yuanhuakine B (2).The anti- dermatophyte activities of the obtained compounds against Microsporum canis,Trichophyton rubrum,Trichophyton mentagrophytes and Malassezia restricta were evaluated by MIC method.The results showed that compounds 1 and 2 displayed certain inhibitory activity against dermatogenic fungi.The inhibitory activities of 1 against T. rubrum and T. mentagrophytes with MIC value 4.0 μg/mL,were equivalent to that of the positive control group,and compound 2 showed significant inhibitory activity against T. rubrum with MIC value 2.0 μg/mL.

Key words: Daphne genkwa, chemical composition, daphnane diterpenoid, anti-dermatophyte activities

中图分类号:  R284.2