天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (增刊1): 54-60.

• 研究简报 • 上一篇    下一篇

不同方法对牛至精油化学成分定性定量分析比较研究

林国栋1,2，李圣杰1,2，程   峰1,2，买吾兰江·买提努尔1,2*   

  1. 1中国科学院新疆理化技术研究所 干旱地区植物资源与化学重点实验室 新疆天然药用植物资源利用国家重点实验室，乌鲁木齐 830011；2中国科学院大学，北京 100039
  • 出版日期:2023-06-28 发布日期:2023-06-13
  • 基金资助:
    中国科学院“西部之光”创新交叉团队项目（2020-JCTD-001）

Comparative study on qualitative and quantitative analysis of chemical components from oregano essential oil by different methods

LIN Guo-dong1,2,LI Sheng-jie1,2,CHENG Feng1,2,MAITINUER Mai-wu-lan-jiang1,2*   

  1. 1The Key Laboratory of Plant Resources and Chemistry of Arid Zone and State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China;2University of the Chinese Academy of Sciences,Beijing 100039,China
  • Online:2023-06-28 Published:2023-06-13

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摘要：

采用气相色谱四级杆-飞行时间-质谱联用（GC-QTOF-MS）搭配三根不同极性毛细管柱分析牛至精油，通过提供更多保留指数指认化合物，提高定性准确度；另外，选择牛至精油中8个含量不同的化合物进行4种定量分析之间差异研究，优化定量准确度。HP-5ms柱中鉴定出24种化合物，HP-1柱中鉴定出33种化合物，HP-INNOWax柱中鉴定出30种化合物，三种极性条件共存的化合物为15种，共鉴定出38种化合物，占精油总含量的97%以上。采用气相色谱四级杆-飞行时间-质谱联用（GC-QTOF-MS）峰面积归一化法、气相色谱-氢火焰离子化（GC-FID）峰面积归一化法、内标法、外标法测定的百里香酚的含量分别为(25.869±0.263)%、(27.567±0.003)%、(7.396±0.068)%、(5.558±0.025)%。多柱分析可以提高牛至精油成分鉴定的准确度；不同定量方法之间各有优劣。

关键词: 多柱定性, 归一化法, 内标法, 标准曲线法, 牛至精油

Abstract:

Analysis of oregano essential oil by gas chromatography quadrupole-time of flight-mass spectrometry (GC-QTOF-MS) with three capillary columns of different polarities to improve the qualitative accuracy by providing more retention indices to identify compounds.In addition,eight compounds with different contents in oregano essential oil were selected for a difference study between four quantitative analyses to optimize the quantitative accuracy.Twenty-four compounds were identified in the HP-5ms column,33 compounds were identified in the HP-1 column,30 compounds were identified in the HP-INNOWax column,and 15 compounds were present simultaneously in the three polar conditions,for a total of 38 compounds,accounting for more than 97% of the total essential oil content.Four methods (GC-QTOF-MS peak area normalization,GC-FID peak area normalization,internal standard method and external standard method) were used to determine the content of thymol,which were (25.869 ± 0.263)%,(27.567 ± 0.003)%,(7.396 ± 0.068)% and (5.558 ± 0.025)%,respectively.

Key words: multi-column qualitative analysis, normalization method, internal standard method, standard curve method, oregano essential oil

中图分类号:  R932

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