天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (6): 915-924.doi: 10.16333/j.1001-6880.2023.6.001

• 研究论文 •    下一篇

响应面法优化金莲花总酚酸的提取工艺及其美白活性研究

杜若君1，石召华1，詹志来2，胡俊杰1*，郑国华1*   

  1. 1湖北中医药大学药学院，武汉 430065；2中国中医科学院中药资源中心，北京 100700
  • 出版日期:2023-06-28 发布日期:2023-06-28
  • 基金资助:
    中国中医科学院中央本级重大增减支项目（2060302）

Optimization of the extraction process of total phenolic acid from Trollii Flos by response surface methodlogy and study on its whitening activity

DU Ruo-jun1,SHI Zhao-hua1,ZHAN Zhi-lai2,HU Jun-jie1*,ZHENG Guo-hua1*   

  1. 1School of Pharmacy,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China;2Traditional Chinese Medicine Resource Center,China Academy of Chinese Medical Sciences,Beijing 100700,China
  • Online:2023-06-28 Published:2023-06-28

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摘要：

采用响应面分析法中Box-Behnken设计实验优化乙醇回流提取金莲花总酚酸的工艺条件，同时研究了金莲花总酚酸对DPPH自由基、羟基自由基的清除能力、还原能力及其对酪氨酸酶的抑制作用。结果表明，金莲花总酚酸的最佳提取工艺为乙醇浓度60%，回流时间90 min，回流温度60 ℃，液料比40∶1（mL/g）；在此条件下金莲花总酚酸得率为92.245 mg/g。此外，其对DPPH自由基、羟基自由基具有一定的清除能力和还原能力，并且能够显著性地抑制酪氨酸酶活性。本研究所得的提取工艺稳定可靠，金莲花总酚酸具有一定的体外抗氧化活性和酪氨酸酶抑制作用，为进一步科学合理开发利用金莲花奠定基础。

关键词: 金莲花, 总酚酸, 响应面, 抗氧化, 酪氨酸酶, 美白

Abstract:

Box-Behnken design experiments in response surface analysis were used to optimize the process conditions for the ethanol reflux extraction of total phenolic acid from Trollii Flos,and the scavenging ability of total phenolic acid from Trollii Flos on DPPH radicals and hydroxyl radicals and its inhibitory effect on tyrosinase were also studied.The results showed that the optimum extraction process of total phenolic acid from Trollii Flos was:ethanol concentration 60%,reflux time 90 min,reflux temperature 60 ℃,liquid to solid ratio 40∶1 (mL/g).The yield of total phenolic acid from Trollii Flos under these conditions was 92.245 mg/g.In addition,it had certain scavenging ability to DPPH radicals and hydroxyl radicals,and could significantly inhibit tyrosinase activity.The extract method is stable and reliable,and the total phenolic acid from Trollii Flos has certain in vitro antioxidant activity and tyrosinase inhibitory effect,which lays the foundation for further scientific and rational development and utilization of Trollii Flos.

Key words: Trollii Flos, total phenolic acid, response surface, antioxidation, tyrosinase, whitening

中图分类号:  TQ28 R284.2

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