天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (7): 1154-1162.doi: 10.16333/j.1001-6880.2023.7.006

• 研究简报 • 上一篇    下一篇

香薷精油成分及其抗氧化、抗菌活性研究

李秋香，董   伟*，赵国巍，徐希强，黄小英，杨   明，梁新丽*   

  1. 江西中医药大学现代中药制剂教育部重点实验室，南昌 330004
  • 出版日期:2023-07-31 发布日期:2023-07-31
  • 基金资助:
    江西省科技厅2019年度重大科技研发专项（20194A BC28009）；国家自然科学基金（82060733）；江西中医药大学校级研究生创新专项基金（JZYC21S38）

Chemical constituents,antioxidant and antibacterial activities of Elsholtzia ciliata essential oil

LI Qiu-xiang,DONG Wei*,ZHAO Guo-wei,XU Xi-qiang,HUANG Xiao-ying,YANG Ming,LIANG Xin-li*   

  1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China
  • Online:2023-07-31 Published:2023-07-31

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摘要：

研究香薷精油（Elsholtzia ciliata essential oil，ECEO）的化学成分及其抗氧化与抗菌活性。水蒸气蒸馏法提取香薷精油，结合气相色谱-质谱联用分析（gas chromatography-mass spectrometry，GC-MS）和傅里叶变换红外光谱分析（Fourier transform infrared spectroscopy，FTIR）对香薷精油的成分进行分析；采用DPPH法和β-胡萝卜素漂白法评价香薷精油的抗氧化能力；测定香薷精油最低抑菌浓度（MIC）和最低杀菌浓度（MBC）及对由铜绿假单胞菌（Pseudomonas aeruginosa）感染引起小鼠肺炎模型的影响。结果表明香薷精油的主要成分为百里香酚（79.17%）和4-异丙基-3-甲基苯酚（12.35%）等，其对DPPH清除能力和β-胡萝卜素抑制率的IC50分别为2.05 μg/mL和6.93 μg/mL。香薷精油对敏感菌和耐药菌均有抑制效果，可有效改善铜绿假单胞菌感染引起的小鼠肺炎，并预防和延缓耐左氧氟沙星的铜绿假单胞菌重复感染引起的小鼠肺炎的发生。以上结果表明，香薷精油具有较强的抗氧化和抗菌的生物活性。

关键词: 香薷精油, 化学成分, 抗氧化, 抗菌, 耐药

Abstract:

To study the chemical composition,antioxidant and antimicrobial activities of Elsholtzia ciliata essential oil (ECEO).ECEO was extracted by steam distillation method,and the chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS) and fourier transform infrared spectroscopy (FTIR).The antioxidant ability of ECEO was evaluated by DPPH and β-carotene bleaching methods. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of ECEO was determined,the effect of ECEO on the pneumonia model infected by P. aeruginosa was studied.The results showed that the main components of ECEO were thymol (79.17%) and 3-methyl-4-isopropylphenol (12.35%),the IC50 of DPPH scavenging ability and β-carotene oxidation inhibition ability were 2.05 and 6.93 μg/mL,respectively.The essential oil showed obvious inhibitory effect on both sensitive and resistant bacteria,and it could also had an effective remission effect on pneumonia induced by P. aeruginosa infection,in addition,ECEO could prevent or postpone the development of pneumonia in mice caused by levofloxacin resistant P. aeruginosa superinfection.The results suggested that ECEO had superior antioxidant activity and broad-spectrum antibacterial effects.

Key words: Elsholtzia ciliata, antibacterial, chemical composition, antioxidant, drug resistance

中图分类号:  R284.2 R285

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