Based on the differences of 60 multiple active constituents in different parts of Tetrastigma hemsleyanum Diels et Gilg (THDG),a comprehensive evaluation of the quality of different parts was performed.The content of 60 active constituents in the tubers,stems and leaves of THDG were determined by ultra-fast high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry.The results of content determination showed that the distribution pattern of the total content of 60 active constituents in different parts was leaf > stem > tuber,reaching 14 263,11 583 and 4 603 μg/g,respectively;among them,the distribution pattern of flavonoids,phenolic acids,amino acids and nucleosides in different parts was the same as 60 active constituents,accounting for 55%-97% of the total measured constituents,while tannins and polyphenols were higher in the stem than in the other two parts;principal component analysis (PCA) showed that the samples of different parts were clearly classified with large differences;partial least squares discriminant analysis (PLS-DA) and one-way analysis of variance (ANOVA) showed that the eight active constituents could be used as indicator constituent markers to distinguish different parts of THDG;the gray correlation results showed that the ranking of different parts of THDG was leaf > stem > tuber,and the quality of the leaf part of THDG was better than the other two parts.The results of this experiment can provide a methodological reference for the rapid identification and quality control of multiple active constituents in different parts of THDG,and also lay the foundation for the rational exploitation of its resources.