天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (8): 1393-1401.doi: 10.16333/j.1001-6880.2023.8.012

• 开发研究 • 上一篇    下一篇

基于UPLC特征图谱和一测多评法的马鞭草质量评价及其标准汤剂量值传递规律研究

杨晓东，罗宇琴，吴文平，潘礼业，刘晓琳，庞   伟，邢菊玲，李国卫*   

  1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
  • 出版日期:2023-08-28 发布日期:2023-08-30
  • 基金资助:
    佛山市应急科技攻关专项（2020001000206）；国家工业和信息化部2019年产业技术基础公共服务平台项目（2019-00902-1-2）

Quality evaluation of Verbenae Herba based on UPLC characteristic chromatogram and QAMS and the transmission law of quantity value of its standard decoction

YANG Xiao-dong,LUO Yu-qin,WU Wen-ping,PAN Li-ye,LIU Xiao-lin,PANG Wei,XING Ju-lin,LI Guo-wei*   

  1. Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China
  • Online:2023-08-28 Published:2023-08-30

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摘要：

建立马鞭草UPLC特征图谱和5-羟基马鞭草苷、马鞭草苷及毛蕊花糖苷含量的一测多评分析方法。对不同产地18批马鞭草进样分析，共确定5个特征共有峰，指认其中3种成分，18批样品相似度均大于0.830。一测多评法与外标法测定结果比较，误差均小于3%，18批标准汤剂中5-羟基马鞭草苷、马鞭草苷及毛蕊花糖苷的含量范围分别为17.36~51.34 mg/g、7.76~72.85 mg/g、1.15~15.44 mg/g；18批马鞭草饮片到标准汤剂5-羟基马鞭草苷、马鞭草苷及毛蕊花糖苷的转移率分别为35.0%~113.4%、52.1%~109.2%、7.9%~36.3%；所建立的特征图谱结合一测多评含量测定法简便可行，研究结果可为马鞭草及马鞭草中药制剂质量评价提供参考。

关键词: 马鞭草, 超高效液相色谱, 特征图谱, 一测多评法, 量值传递

Abstract:

To establish the UPLC characteristic chromatogram of Verbenae Herba and quantitative analysis of multi-components by single marker(QAMS) for the hastatoside,cornin and verbascoside,a total of five characteristic common peaks were identified and three components were identified for the analysis of 18 batches of Verbenae Herba from different habitats.The similarity of 18 batches of samples was greater than 0.830.The errors were all less than 3% when compared with the results measured by the external standard method.hastatoside,cornin and verbascoside in 18 batches of standard decoction were 17.36-51.34 mg/g,7.76-72.85 mg/g,1.15-15.44 mg/g.The transfer rates of 18 batches of Verbenae Herba to standard decoction hastatoside,cornin and verbascoside were 35.0%-113.4%,52.1%-109.2%,7.9%-36.3%.The simple and feasible established characteristic chromatogram combined with QAMS can provide reference and basis for the overall quality control of Verbenae Herba.

Key words: Verbenae Herba, UPLC, characteristic chromatogram, QAMS, quantity value transmission

中图分类号:  R282.4

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