天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (10): 1716-1723.doi: 10.16333/j.1001-6880.2023.10.007

• 研究简报 • 上一篇    下一篇

五岭龙胆化学成分及其抗炎活性研究

彭江丽1,2,3，王   鹏1，彭求贤1，李   娟1,2,3*，李顺祥1,2,3*   

  1. 1湖南中医药大学药学院；2湖南省中药活性物质筛选工程技术研究中心；3湖南省中美老年性退行性疾病治疗药物国际联合研究中心，长沙410208
  • 出版日期:2023-10-28 发布日期:2023-10-31
  • 基金资助:
    国家自然科学基金面上项目（81973593）；湖南省自然科学基金-科药联合项目（2022JJ80087）；国家级大学生创新创业训练计划（202210541066）；湖南中医药大学中药学一流学科基金（校行科字［2018］3号）

Chemical constituents and anti-inflammatory activity of Gentiana davidii Franch.

PENG Jiang-li1,2,3,WANG Peng1,PENG Qiu-xian1,LI Juan1,2,3*,LI Shun-xiang1,2,3*   

  1. 1School of Pharmacy,Hunan University of Chinese Medicine;2Hunan Engineering Research Center of Bioactive Substance Discovery of C Chinese Medicine;3Hunan Province Sino-US International Joint Research Center for Therapeutic Drugs of Senile Degenerative Diseases,Changsha 410208,China
  • Online:2023-10-28 Published:2023-10-31

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摘要：

研究五岭龙胆（Gentiana davidii Franch.）的化学成分及其体外抗炎活性。综合应用硅胶柱色谱、反相ODS柱色谱和Sephadex LH-20凝胶柱色谱等分离技术，对五岭龙胆70%乙醇提取物的乙酸乙酯和正丁醇萃取部位进行系统分离纯化，并结合化合物理化性质、波谱数据及与文献数据比对等进行结构鉴定，从中共分离鉴定15个化合物，分别为熊果酸（1）、乌发醇（2）、ursaldehyde（3）、28-norurs-12-en-3β-ol（4）、齐墩果酸（5）、β-amyrin-n-nonyl ether（6）、vanillyl glycol（7）、β-谷甾醇（8）、β-胡萝卜苷（9）和anemarrhenoside B（10）、正二十六烷（11）、正三十一烷醇（12）、1,5-bis β-D-glucopyranosyloxy-2-(3′,3′-dimethylallyl ) benzene（13）、2,4-二叔丁基苯酚（14）和2,2′-oxybis(1,4-di-tert-butylbenzene)（15）。化合物7、14和15为龙胆属首次分离得到，所有化合物均为五岭龙胆首次分离得到。对化合物1~15进行抗炎活性筛选发现，与模型组比较，化合物1、5和9对脂多糖诱导的RAW 264.7细胞释放的NO有显著抑制作用。

关键词: 五岭龙胆, 化学成分, 抗炎活性, 三萜, 苯丙素

Abstract:

This study aimed to investigate the chemical constituents and anti-inflammatory activity in vitro from Gentiana davidii Franch..The chemical constituents of the ethyl acetate and n-butanol fractions of 70% ethanol extract in G. davidii were separated and purified by silica gel,ODS gel,and Sephadex LH-20 gel column chromatographies.Their structures were identified by physicochemical properties,spectroscopic analysis,as well as comparisons with the data reported in literature.Fifteen compounds were isolated and identified as ursolic acid (1),uvaol (2),ursaldehyde (3),28-norurs-12-en-3β-ol (4),oleanolic acid (5),β-amyrin-n-nonyl ether (6),vanillyl glycol (7),β-sitosterol (8),β-daucosterol (9),anemarrhenoside B (10),n-hexacosane (11),n-hentriacontyl alcohol (12),1,5-bis β-D-glucopyranosyloxy-2-(3′,3′-dimethylallyl)benzene (13),2,4-di-tert-butylphenol (14),2,2′-oxybis(1,4-di-tert-butylbenzene) (15).Compounds 7,14 and 15 were first isolated from Gentiana.All the compounds were first isolated from G. davidii. The compounds 1-15 were preliminarily examined for anti-inflammatory activity in vitro. Compared with the model group,compounds 1,5 and 9 significantly inhibited the production of NO in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Key words: Gentiana davidii Franch., chemical composition, anti-inflammatory activity, triterpenes, phenylpropanoids

中图分类号:  R284.2

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