天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (增刊2): 76-83.

• 开发研究 • 上一篇    下一篇

唐古特大黄非药用部位多糖脱色工艺及其抗氧化活性研究

冯   丹1,2,龙若兰1,2,李佩佩1,2,李   朵1,2,孙菁1*   

  1. 1中国科学院西北高原生物研究所 青海省青藏高原特色生物资源研究重点实验室,西宁 810008;2中国科学院大学,北京 100049
  • 出版日期:2023-12-27 发布日期:2023-12-11
  • 基金资助:
    青海省科研基础条件平台项目(2020-ZJ-T05);中国科学院仪器设备功能开发技术创新项目(2022gl09);青海省重点实验室建设专项(2022-ZJ-Y18)

Study on decolorization technology and antioxidant activity of polysaccharides from non-medicinal parts of Rheum tanguticum

FENG Dan1,2,LONG Ruo-lan1,2,LI Pei-pei1,2,LI Duo1,2,SUN Jing1 *   

  1. 1Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resource,Northwest Institute of Plateau Biology, Chinese Academy of Sciences,Xining 810008,China;2University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2023-12-27 Published:2023-12-11

摘要:

本文分别选取了3种活性炭、3种大孔吸附树脂和氨基氧化石墨烯等三类脱色材料对唐古特大黄非药用部位多糖溶液进行脱色工艺研究,比较三类脱色剂下的多糖脱色率及保留率,并采用DPPH法测定其脱色后多糖的抗氧化能力。结果显示,在只考虑脱色率与保留率的情况下,选择活性炭为最佳脱色剂,脱色率与保留率分别为79.59%与88.72%。最佳脱色条件为:200目活性炭,pH=5.0,活性炭用量与多糖溶液体积比1∶100,保温时间20 min,保温温度60 ℃。然而,经过活性炭处理后的样品的抗氧化效率降低,而石墨烯与树脂处理后的样品抗氧化活性则提高。因此,在优先选择抗氧化活性的情况下,考虑石墨烯作为最佳脱色剂,应根据需要特定地选择脱色剂与评价指标。

关键词: 唐古特大黄非药用部位, 多糖, 脱色工艺, 抗氧化活性

Abstract:

Rheum tanguticum is a tall perennial herb of Rheum Linn.in Polygonaceae,and its non-medicinal parts have important development and utilization value,a certain amount of polysaccharides are stored in the stems and leaves.Commonly used decolorization methods of polysaccharides include activated carbon method,macroporous resin adsorption method,etc.Graphene has been widely used in recent years because of its strong adsorption capacity.In this paper,three kinds of activated carbon,three kinds of macroporous adsorption resins and amino graphene oxide were selected to discuss the decolorization technology of polysaccharide solution from the non-medicinal parts of R. tanguticum.Then,the decolorization rate and retention rate of polysaccharide under three kinds of decolorizing agents were compared.Meanwhile,the antioxidant capacity of decolorized polysaccharide was determined by DPPH method.The results showed that activated carbon was the best decolorizing agent when only the decolorization rate and retention rate were considered,the decolorization rate and retention rate were 79.59% and 88.72% respectively.The optimum decolorization conditions were as follows:200 mesh activated carbon,pH=5.0,volume ratio of activated carbon∶polysaccharide solution=1∶100,soaking time 20 min,temperature 60 ℃.However,the antioxidant efficiency of the samples treated with activated carbon was the lowest,while the antioxidant activity of the samples treated with graphene and resin was improved.Therefore,in the case of giving priority to antioxidant activity,considering graphene as the best decolorizing agent.As a result,according to the different needs of decolorization,the decolorizing agent and evaluation index should be specifically selected.

Key words: Rheum tanguticum medicinal part, polysaccharise, decolorization process, antioxidant activity

中图分类号:  R282.5