天然产物研究与开发 ›› 2023, Vol. 35 ›› Issue (增刊2): 164-173.

• 综述 • 上一篇    下一篇

基于黄芪毛状根技术的次生代谢产物研究进展

曹文颖1,2,徐冰艳1,2,彭国情3,陈   银1,2,黄建萍4,黄胜雄1*   

  1. 1中国科学院昆明植物研究所 植物化学与西部资源持续利用国家重点实验室,昆明 650201;2中国科学院大学,北京 100049; 3成都中医药大学药学院;4成都中医药大学中医药创新研究院,成都 611137
  • 出版日期:2023-12-27 发布日期:2023-12-12
  • 基金资助:
    云南省云岭学者研究项目和中国科学院先导B专项(XDB27020205)

Research progress of secondary metabolites based on Astragalus membranaceus hairy root technique#br#
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CAO Wen-ying1,2,XU Bing-yan1,2,PENG Guo-qing3,CHEN Yin1,2,HUANG Jian-ping4,HUANG Sheng-xiong1*   

  1. 1State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China; 2University of Chinese Academy of Sciences,Beijing 100049,China;3School of Pharmacy,Chengdu University of Traditional Chinese Medicine;4 Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China
  • Online:2023-12-27 Published:2023-12-12

摘要: 黄芪为我国常用中药,主要活性成分包括异黄酮、多糖及三萜皂苷等,其中黄芪多糖和黄芪甲苷已开发为兽用抗病毒药物。由于黄芪及其活性化合物市场需求量的增加,探寻植物提取的替代途径愈发重要。毛状根是植物生产活性次级代谢产物的潜在替代途径,同时是研究植物次级代谢产物生物合成途径的重要体系,该技术在黄芪相关研究中也早有应用。本文从黄芪毛状根体系的建立和优化、不同诱导子对毛状根生长和活性成分含量的影响、毛状根与栽培黄芪提取物对比、转基因技术提高毛状根中黄芪甲苷含量,以及基于毛状根的黄芪皂苷生物合成途径研究五个方面对黄芪毛状根相关研究进行综述。

关键词: 中药黄芪, 黄芪, 毛状根, 诱导子, 次级代谢产物

Abstract:

Astragali Radix is a commonly used traditional Chinese medicine whose main active compounds include isoflavones,polysaccharides and triterpene saponins,among which astragalus polysaccharide and astragaloside Ⅳ have been developed as veterinary antiviral drugs.Due to the rising market demand for Astragali Radix and its active compounds,it is increasingly important to explore alternative ways of plant extraction.Hairy root provides a potential alternative source for production of active secondary metabolites in plant and it is an important system to study the biosynthesis pathway of plant secondary metabolites.This technique has been used in the research of A. membranaceus for a long time.In this paper,studies related to hairy root of A. membranaceus were reviewed from five aspects:establishment and optimization of hairy root system,effects of different elicitors on hairy root growth and active component content,comparison of hairy root extract with cultivated plant extract,transgenic technology to increase the content of astragaloside Ⅳ in hairy root,and studies on astragalosides biosynthetic pathways based on hairy roots.

Key words: Astragali Radix, Astragalus membranaceus, hairy root, elicitor, secondary metabolites

中图分类号:  R932