天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (1): 13-25.doi: 10.16333/j.1001-6880.2024.1.002

• 研究论文 • 上一篇    下一篇

黄姜花花的化学成分、抗氧化、酶抑制活性及抗炎作用研究

丁芙蓉1,3，贾小演1,3，吴相欢1,3，吴   霞1,3，杨   念2,3，刘雄利1,3，田民义1,3*


  

  1. 1贵州大学酿酒与食品工程学院；2贵州大学生命科学学院；3西南药食两用资源开发利用技术国家地方联合工程研究中心，贵阳 550025
  • 出版日期:2024-01-23 发布日期:2024-01-23
  • 基金资助:
    国家自然科学基金（82360834）

Chemical composition,antioxidant,enzyme inhibitory and anti-inflammatory activities of Hedychium flavum flower

DING Fu-rong1,3,JIA Xiao-yan1,3,WU Xiang-huan1,3,WU Xia1,3,YANG Nian2,3,LIU Xiong-li1,3,TIAN Min-yi1,3*   

  1. 1School of Liquor and Food Engineering,Guizhou University;2College of Life Sciences,Guizhou University;3National & Local Joint Engineering Research Center for the Exploitation of Homology Resources of Southwest Medicine and Food,Guiyang 550025,China
  • Online:2024-01-23 Published:2024-01-23

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摘要： 研究黄姜花花提取物的化学成分、抗氧化、酶抑制活性及抗炎作用。本文采用回流提取法制得黄姜花花水提物和醇提物，使用超高效液相色谱-四极杆静电场轨道阱串联质谱（UHPLC-Q-Orbitrap MS）分析其提取物化学成分，共鉴定出58种化合物，包括11种酚类和18种黄酮类化合物。黄姜花花提取物具有较好的抗氧化活性，对DPPH、ABTS自由基的清除作用较强，同时能够抑制α-葡萄糖苷酶、酪氨酸酶、乙酰胆碱酯酶和丁酰胆碱酯酶的活性。提取物在无毒范围内（62.5、125、250 μg/mL）能显著降低LPS诱导的RAW 264.7细胞中促炎症介质（NO和PGE2）和细胞因子（TNF-α、IL-6和IL-1β）的过度分泌。本实验为黄姜花花在食品、药品、化妆品等行业的开发利用提供理论基础。

关键词: 黄姜花, 提取物, 化学成分, 抗氧化活性, 酶抑制活性, 抗炎活性

Abstract:

This study aimed to investigate the chemical composition,antioxidant,enzyme inhibitory,and anti-inflammatory activities of Hedychium flavum flower extracts.H. flavum flower water extracts and ethanol extracts were obtained by reflux extraction method.The chemical composition of the extracts was identified by ultra-high-performance liquid chromatography coupled to quadrupole-orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS).A total of 58 compounds were identified,including 11 phenols and 18 flavonoids.The extracts showed strong antioxidant activity in the DPPH and ABTS assay and had certain inhibitory effects on α-glucosidase,tyrosinase,acetylcholinesterase,and butyrylcholinesterase.Within the non-toxic dose range (62.5,125,250 μg/mL),the extracts significantly reduced LPS-induced oversecretion of pro-inflammatory mediators (NO and PGE2) and cytokines (TNF-α,IL-6,IL-1β) in RAW 264.7 cells.Our study provides a theoretical basis for the development and utilization of H. flavum flower in the food,pharmaceutical,and cosmetic industries.

Key words: Hedychium flavum, extracts, chemical composition, antioxidant activity, enzyme inhibition activity, anti-inflammatory activity

中图分类号:  R285.5

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