天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (1): 102-108.doi: 10.16333/j.1001-6880.2024.1.012

• 开发研究 • 上一篇    下一篇

基于响应面法优化牛蒡子多糖提取工艺及对细胞抗炎作用研究

潘博雅，孟宪群，康廷国，尹海波，许   亮，王   丹*   

  1. 辽宁中医药大学药学院，大连 116600
  • 出版日期:2024-01-23 发布日期:2024-01-23
  • 基金资助:
    国家自然科学基金（81874338）；沙河口区、望花区中药资源普查基金（2019008）；2021年辽宁中医药大学大学生创新创业训练计划（X202110162027）

Optimization of extraction process of Arctii Fructus polysaccharide based on response surface method and its anti-inflammatory effect on cells

PAN Bo-ya,MENG Xian-qun,KANG Ting-guo,YIN Hai-bo,XU Liang,WANG Dan*   

  1. College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China
  • Online:2024-01-23 Published:2024-01-23

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摘要： 为了确定提取牛蒡子多糖的最佳工艺条件，并探究其抗炎作用。通过响应面法考察不同料液比，提取时间和提取温度对牛蒡子多糖提取率的影响；使用ELISA试剂盒测定不同RAW 264.7细胞处理组肿瘤坏死因子α（TNF-α）和白介素-6（IL-6）的水平；经Western blot法检测髓样分化因子88（MyD88）、Toll样受体4（TLR4）和核转录因子-κB（NF-κB）的蛋白表达水平。结果显示牛蒡子多糖最优提取工艺条件为料液比1∶15（g/mL）、提取时间3 h、提取温度80℃，此时提取率为7.19%；牛蒡子多糖组对TNF-α和IL-6的分泌均有抑制作用并通过调控相关通路上的蛋白表达，起到免疫调控作用。该研究可为牛蒡子新药用成分的开发和应用奠定基础。

关键词: 牛蒡子多糖, 响应面法, 提取工艺, RAW 264.7细胞, 抗炎

Abstract:

The aim of this paper was to determine the optimum extraction conditions of Arctii Fructus polysaccharide and to explore the anti-inflammatory effect of its solution.The effects of different solid-liquid ratio,extraction time and extraction temperature on the extraction yield of Arctii Fructus polysaccharide were investigated by response surface method.The levels of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) in different RAW 264.7 cell treatment groups were determined by ELISA kit.The protein expression levels of myeloid differentiation factor 88 (MyD88),Toll-like receptor 4 (TLR4) and nuclear transcription factor-κB (NF-κB) were detected by Western blot.The results showed that the optimal extraction conditions were solid-liquid ratio 1∶15 (g/mL),extraction time 3 h,extraction temperature 80 ℃,and the extraction yield was 7.19%.Arctii Fructus polysaccharide groups inhibited the secretion of TNF-α and IL-6,and played an immunoregulatory role by regulating the expression of proteins in related pathways.This study can lay a foundation for the development and application of new drug ingredients of Arctii Fructus.

Key words: Arctii Fructus polysaccharides, response surface methodology, extraction process, RAW 264.7 cells, anti-inflammatory

中图分类号:  R932

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