天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (6): 982-985.doi: 10.16333/j.1001-6880.2024.6.008

• 研究简报 • 上一篇    下一篇

怀山药内生真菌Chaetomium sp.的次生代谢产物研究

盛蒙珂1,赵莹菲1,田若丹1,文春南1,2,刘   淼1,2*,麻兵继1,2*   

  1. 1河南农业大学农学院;2郑州市中药材种质资源创新与代谢工程重点实验室,郑州 450046
  • 出版日期:2024-06-26 发布日期:2024-06-26
  • 基金资助:
    国家自然科学基金(U1604169);河南省自然科学基金(232300421127);河南农业大学博士启动基金(30500662)

Secondary metabolites from the endophytic Chaetomium sp. of Dioscorea opposita

SHENG Meng-ke1,ZHAO Ying-fei1,TIAN Ruo-dan1,WEN Chun-nan1,2,LIU Miao1,2 *,MA Bing-ji1,2 *   

  1. 1College of Agronomy,Henan Agricultural University;2Zhengzhou Key Laboratory of Germplasm Resources Innovation and Metabolic Engineering of Traditional Chinese Medicine,Henan Agricultural University,Zhengzhou 450046,China
  • Online:2024-06-26 Published:2024-06-26

摘要:

以一株怀山药内生毛壳菌Chaetomium sp.L-45为研究对象,对其次生代谢产物进行分离和结构鉴定。对毛壳菌进行大米发酵,通过硅胶柱色谱、Sephadex LH-20、半制备高效液相色谱等分离技术分离发酵物的乙酸乙酯提取物,利用NMR、HR-ESI-MS等波谱解析技术进行化合物的结构鉴定。从目标菌株的乙酸乙酯提取物中分离得到4个化合物,分别鉴定为3,4-二甲氧基-5-羟基-6-丙基-苯甲酸(1)、5-异戊烯基-吲哚-3-羧酸(2)、lecanorin(3)和麦角甾醇(4),其中,化合物1为新化合物,化合物23系首次从毛壳菌属真菌中分离得到。

关键词: 毛壳菌, 次生代谢产物, 提取分离, 结构鉴定

Abstract:

In this study,the secondary metabolites of an endophytic Chaetomium sp.L-45 from Dioscorea opposita were isolated and identified.The ethyl acetate extract of the rice fermentation of the fungus were separated by silica gel column chromatography,Sephadex LH-20,semi-preparative High-performance liquid chromatography,etc.,and the chemical structures of the compounds were identified by NMR and HR-ESI-MS analysis.As a result,four compounds were isolated and identified as 3,4-dimethoxy-5-hydroxy-6-propyl-benzoic acid (1),5-isoprenylindole-3-carboxylic acid (2),lecanorin (3),and ergosterol (4).Among them,compound 1 is a new compound,compounds 2 and 3 were isolated from genus Chaetomium for the first time.

Key words: Chaetomium sp., secondary metabolites, extraction and isolation, structure elucidation

中图分类号:  R284.1 R284.2