In the artificial cultivation of Ganoderma lucidum,different wood segments are often used and there are significant differences in their composition that affect G .lucidum.In order to compare the differences in components,HPLC fingerprints of G .lucidum was performed and the content of ganoderic acid A and B was determined,which provides a reference for the quality evaluation and cultivation techniques of different wood segments of G .lucidum.The analysis was performed on Diamonsil C₁₈ (2) (250 mm× 4.6 mm,5 μm) with acetonitrile-0.1% phosphoric acid solution as the mobile phase in a gradient elution mode,a total of 18 common peaks were calibrated and ganoderic acid A and ganoderic acid B were identified.The similarity of the 15 batches of G .lucidum ranged from 0.601 to 0.986.Principal component analysis divides them into four categories,and orthogonal partial least squares discriminant analysis (OPLS-DA) selects seven differential components with VIP>1.0.The content determination showed that the content of ganoderic acid A and ganoderic acid B in the willow+birch wood segment was 2.29 mg/g and 0.47 mg/g,respectively.Both were the highest,suggesting that their content might be related to the cultivated segment of the wood.This method can be used to evaluate the quality of G .lucidum from different wood segments,screen suitable G .lucidum cultivated wood segments,and guide G .lucidum cultivation.