To establish the HPLC fingerprints of rubbed and unrubbed Hedysari Radix samples,the differences between rubbed and unrubbed samples were compared with multi-component quantification.The chromatographic column was Angilent C₁₈ (4.6 mm × 250 mm,5 μm).The flow rate was 1.0 mL/min,and the mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile (gradient elution).The column temperature was 30 ℃.The fingerprints of 14 batches of rubbed and unrubbed samples were established,through similarity evaluation and chemometric analysis,and the difference between rubbed and unrubbed samples was studied subsequently.The contents of components such as calycosin,formononetin,total polysaccharides,and total flavonoids were determined.The results showed that there were 25 common peaks in the fingerprint of rubbed and unrubbed samples.Seven components were identified as adenosine,vanillic acid,calycosin-7-glucoside,ononin,calycosin,formononetin,medicarpin.Both hierarchical clustering analysis and principal component analysis can be distinguished between rubbed and unrubbed samples.Orthogonal partial least squares-discriminant analysis found twelve components of difference,and the order of significance of difference was peak 25> peak 23> peak 14>formononetin>calycosin-7-glucoside>calycosin> peak 18> peak 19> peak 15>ononin>peak 4>vanillic acid.The results of content determination showed that the content of calycosin,formononetin,total polysaccharide,and extract in rubbed samples was significantly higher than in the unrubbed ones.In contrast,the contents of calycosin-7-glucoside,ononin,vanillic acid,and total flavonoids in rubbed samples were significantly lower than in the unrubbed ones.The study can provide data reference for quality and rubbing processing of Hedysari Radix.