天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (10): 1639-1659.doi: 10.16333/j.1001-6880.2024.10.001

• 研究论文 •    下一篇

基于UPLC-QE Plus-MS/MS技术的广东紫珠化学成分分析及其神经保护活性

刘永林1,2，许   熙1，汤   圆1，曾   聪1，胡   培2，陈   杰1*，李诒光1,2*   

  1. 1江西中医药大学药学院，南昌 330004；2江中药业股份有限公司，南昌 330096
  • 出版日期:2024-10-28 发布日期:2024-10-28
  • 基金资助:
    江西省重点研发计划（20212BBG71007）

Chemical composition analysis of Callicarpa kwangtungensis by UPLC-QE Plus-MS/MS and its neuroprotective activity

LIU Yong-lin1,2,XU Xi1,TANG Yuan1,ZENG Cong1,HU Pei2,CHEN Jie1 *,LI Yi-guang1,2 *   

  1. 1School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;2Jiangzhong Pharmaceutical Co.,Ltd.,Nanchang 330096,China
  • Online:2024-10-28 Published:2024-10-28

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摘要：

分析广东紫珠（Callicarpa kwangtungensis，CK）的化学成分并探讨其对神经细胞的保护作用。本研究采用超高效液相色谱串联四级杆-静电场轨道阱高分辨质谱（UPLC-QE Plus-MS/MS）对CK的化学成分进行鉴定。通过建立体外神经炎症模型，检测一氧化氮（nitric oxide，NO）、白细胞介素-1β（interleukin-1β，IL-1β）和白细胞介素-6（interleukin-6，IL-6）炎症因子含量，结合细胞流式术检测活性氧（reactive oxygen species，ROS）水平，并使用免疫荧光观察细胞形态变化，以评估CK的神经保护作用。在CK中共鉴定出108个化学成分，包括34个苯乙醇苷类、10个苯丙素类、25个黄酮类、23个萜类、10个有机酸类、2个有机醛类和4个其他类化合物，其中21个成分为首次鉴定。体外实验结果表明，CK能够抑制脂多糖诱导的BV2细胞NO、IL-1β、IL-6和ROS的表达水平，并能够逆转BV2细胞的活化状态。本研究通过UPLC-QE Plus-MS/MS实现对CK化学成分进行快速分析，并探讨了其神经保护活性，为后续的药效物质基础研究和新药开发提供了重要的理论依据。

关键词: 广东紫珠, 化学成分, UHPLC-QE Plus-MS/MS, 神经炎症, 神经保护

Abstract:

This study aims to analyze the chemical constituents of Callicarpa kwangtungensis (CK) and investigate its neuroprotective activity.The chemical constituents of CK were identified by UPLC-QE Plus-MS/MS. By establishing an in vitro neuroinflammation model,the levels of nitric oxide (NO),interleukin-1β (IL-1β),and interleukin-6 (IL-6) inflammatory factors were detected,and the levels of reactive oxygen species (ROS) were measured in combination with flow cytometry,and changes in cell morphology were observed using immunofluorescence to assess the neuroprotective effects of CK.A total of 108 compounds were identified in CK,including 34 phenylethanoid glycosides,10 phenylpropanoids,25 flavonoids,23 terpenoids,10 organic acids,2 organic aldehydes,and 4 others,of which 21 components were identified for the first time.The results of in vitro experiments showed that CK was able to inhibit lipopolysaccharide-induced expression levels of NO,IL-1β,IL-6,and ROS in BV2 cells and reverse the activation state of BV2 cells. In this study,the rapid analysis of the chemical components of CK was achieved by UPLC-QE Plus-MS/MS,and its neuroprotective activity was investigated,which provided an important theoretical basis for subsequent basic research on pharmacophore substances and the development of new drugs.

Key words: Callicarpa kwangtungensis, chemical composition, UHPLC-QE Plus-MS/MS, neuroinflammation, neuroprotection

中图分类号:  R284.1 R285.5

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