魔芋神经酰胺的提取及其鞘磷脂含量的测定

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 542-546.

魔芋神经酰胺的提取及其鞘磷脂含量的测定

刘仁萍1，杨建奎2，颜焱娜1，吴民熙1，肖鹜轩3，吴永尧1*

1湖南农业大学生物科学技术学院；2湖南农业大学理学院；3湖南省农业科学研究院，长沙 410128

收稿日期:2009-09-07 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: yywu357@sohu.com
基金资助:
湖南省科技计划重大专项（04NK1002）

Extraction of Ceramide from Konjac and Its Sphingomyelin Analysis

LIU Ren-ping1, YANG Jian-kui2, YAN Yan-na1, WU Min-xi1, XIAO Wu-xuan3, WU Yong-yao1*

1College of Bioscience and Biotechnology, Hunan Agriculture University; 2Science of College of Hunan Agriculture University; 3Hunan Academy of Agriculture sciences, Changsha 410128, China

Received:2009-09-07 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

采用回流提取法提取魔芋神经酰胺进行初步的探索，重点分析了溶剂浓度、温度、时间、料液比对魔芋神经酰胺提取量的影响，确定了最佳的提取工艺；并且结合高效液相/蒸发光散射检测器的方法对魔芋结合态神经酰胺－鞘磷脂进行了定性定量分析。

关键词: 魔芋, 鞘磷脂, 高效液相色谱-蒸发光散射器

Abstract:

The extration of ceramide from konjac by the refluxing method was studied. The effects of temperature, time, proportion of solute to solvent and extracting solvent on the extraction efficiencies were analyzed. The optimum extraction conditions were made. Combined with HPLC and evaporative light scattering detector (HPLC/ELSD), The sphingomyelin of konjac were analyzed qualitatively and quantitatively.

Key words: konjac, sphingomyelin, HPLC-ELSD

中图分类号:

Q503

刘仁萍，杨建奎，颜焱娜，吴民熙，肖鹜轩，吴永尧. 魔芋神经酰胺的提取及其鞘磷脂含量的测定[J]. 天然产物研究与开发, 2011, 23(3): 542-546.

LIU Ren-ping, YANG Jian-kui, YAN Yan-na, WU Min-xi, XIAO Wu-xuan, WU Yong-yao. Extraction of Ceramide from Konjac and Its Sphingomyelin Analysis[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 542-546.

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