同时提取光果甘草中光甘草定和甘草酸的工艺研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 547-550.

同时提取光果甘草中光甘草定和甘草酸的工艺研究

李玉会，徐德平，张连富*

江南大学食品学院，无锡 214122

收稿日期:2010-05-13 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: zhanglianfu@hotmail.com
基金资助:
科技部“十一五”国家科技支撑计划（2006BAD27B03）

Simultaneous Extraction of Glabridin and Glycyrrhizic Acid from Glycyrrhiza glabra L.

LI Yu-hui, XU De-ping, ZHANG Lian-fu*

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2010-05-13 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

本文主要对光果甘草中光甘草定、甘草酸两种重要活性成分的同时提取工艺进行了研究。通过单因素试验确定了影响提取的主要因素及适宜水平范围，通过正交试验确定了其最佳提取条件为：用含0.6%氨水的60%乙醇溶液作为提取剂，料液比为1:20（w:v），在75 ℃条件下提取2 h。最佳提取条件下光甘草定的得率为0.238±0.002%，甘草酸的得率为5.08±0.03%。该工艺操作简单，投入一批原料，即可同时提取其中的光甘草定和甘草酸，为实现甘草资源高附加值的综合加工利用奠定了基础。

关键词: 光果甘草, 光甘草定, 甘草酸, 提取

Abstract:

To study the technology of simultaneous extraction of glabridin and glycyrrhizic acid from Glycyrrhiza glabra L., the single factor experiments were done to determine the influencing factors and the orthogonal tests were done to optimize the extracting condition. The extraction conditions were optimized as follows: 60% ethanol containing 0.6% ammonia as extractant, ratio of material to liquid 1:20 (w:v), extraction temperature 75 ℃, and time 2 h. Under these conditions, the extraction rates of Glabridin and Glycyrrhizic acid were 0.238±0.002% and 5.08±0.03% respectively. This technology featured simple operation and yielded the two important bioactive compounds at the same time. The research provided a basis for comprehensive development and utilization of Glycyrrhiza resources with high valued-added.

Key words: Glycyrrhiza glabra L., glabridin, glycyrrhizic acid, extraction

中图分类号:

TS202.3 R282.4 R284.2

李玉会，徐德平，张连富. 同时提取光果甘草中光甘草定和甘草酸的工艺研究[J]. 天然产物研究与开发, 2011, 23(3): 547-550.

LI Yu-hui, XU De-ping, ZHANG Lian-fu. Simultaneous Extraction of Glabridin and Glycyrrhizic Acid from Glycyrrhiza glabra L.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 547-550.

参考文献 12

1	Bai FM(白凤梅). Function and main properties of Glycyrhizin.Global Food Ind(中外食品),2003, (1):13.
2	Wang ZL(王照兰),Du JC(杜建材),Yu LQ(于林清),et al.The utilization value, current research and problems of Glycyrrhiza.Grassland China(中国草地),2002,24(1):73-75.
3	Vaya J,Belinky PA,Aviram M.Antioxidant constituents from licorice roots: isolation structure elucidation and antoxidative capacity toward LDL oxidation.Free Radic Biol Med,1997,23:302-313.
4	Fuhrman B,Vol kova N,Kaplan M,et al.Antiatherosclerotic effects of licorice extract supplementation on hypercholesterolemic patients: increased resistance of LDL to atherogenic modifications, reduced plasma lipid levels,and decreased systolic blood pressure.Nutrition,2002,18:268-273.
5	Mira R,Paula B,Jacob V,et al.Macrophage enrichment with the isoflavan glabridin inhibits NADPH oxidase-induced cell-mediated oxidation of low density lipoprotein.J Biol Chem,1999,274:13790-13799.
6	Aoki F,Nakagawa K,Kitano M,et al.Clinical safety of licorice flavonoid oil (LFO) and pharmacokinetics of glabridin in healthy humans.J Am Coll Nutr,2007,26(3):209-218.
7	Fuhrman B,Bush S,Vaya J,et al.Licorice extract and its major polyphenol glabridin protect low-density lipoprotein against lipid peroxidation:in vitro and ex vivo studies in humans and in atherosclerotic apolipoprotein E-deficient mice.Am J Clin Nutr,1997,66:267-275.
8	Okada K,Tamura Y,Yamamoto M,et al.Identification of antimicrobial and antioxidant constituents from licorice of Russian and Xinjiang origin.Chem Pharm Bull,1989,37:2528-2530.
9	Mourboul A(木合布力·阿布力孜), Rena K(热娜·卡斯木), Ma SY(马淑燕),et al.Isolation and antioxidant property of glabridin.Nat Prod Res Dev(天然产物研究与开发),2007,19:675-682.
10	Shanker K,Fatima A,Negi AS,et al.RP-HPLC method for the quantitation of glabridin in Yashti-madhu (Glycyrrhiza glabra).Chromatographia,2007, 65:771-774.
11	Li J(李佳),Song XB(宋新波),Yu BL(余保林),et al.Content determination of glabridin in G. glabra L. with HPLC. Tianjin J Tradit Chin Med(天津中医药), 2008, 25(2):157-158.
12	Chinese Pharmacopoeia Commission(国家药典委员会).Pharmacopoeia of the People's Republic of China (中国药典), Vol. I. Beijing: Chemical Industry Press, 2005.59-60.

[1]	张亚梅, 韩鸿萍, 宋道光. 响应面法优化暗紫贝母流浸膏提取工艺及其质量评价[J]. 天然产物研究与开发, 2024, 36(9): 1554-1563.
[2]	丁亚男, 王瑞阳, 高观祯, 汪惠勤, 周建武, 饶平凡. 油橄榄叶提取物对HaCaT细胞氧化损伤的保护作用及抑菌活性[J]. 天然产物研究与开发, 2024, 36(9): 1584-1591.
[3]	黄丽荣, 余佳, 李娇, 程莎骆衡. 基于网络药理学及实验验证探究苗药虎掌草不同溶剂提取物抗前列腺癌活性及其作用机制[J]. 天然产物研究与开发, 2024, 36(7): 1231-1241.
[4]	盛蒙珂, 赵莹菲, 田若丹, 文春南, 刘淼, 麻兵继. 怀山药内生真菌Chaetomium sp.的次生代谢产物研究[J]. 天然产物研究与开发, 2024, 36(6): 982-985.
[5]	李百元, 张叶敏, 夏晶晶, 姜红宇, 曹林艳, 陈丹. 19种中药提取物对海洋致病溶藻弧菌生长和生物被膜的影响[J]. 天然产物研究与开发, 2024, 36(6): 1013-1021.
[6]	宋钰, 纪丽莲, 刘艳丽, 王桃云. 枸骨的化学成分及其药理活性研究进展[J]. 天然产物研究与开发, 2024, 36(6): 1082-1099.
[7]	李超, 刘云华, 秦登云, 黄志芳, 刘玉红, 陈燕, 易进海. 基于正交设计优化新鲜川芎中挥发油提取工艺[J]. 天然产物研究与开发, 2024, 36(5): 771-778.
[8]	张宏岐, 朱烈琴, 曾亚妮, 崔敏, 周莹霞, 杜娟. 忍冬木层孔菌多糖的提取工艺优化及其体外抗氧化活性研究[J]. 天然产物研究与开发, 2024, 36(4): 581-588.
[9]	邓超凡, 江志波, 马晓莉, 南泽东. 紫堇属植物苄基异喹啉类成分研究进展[J]. 天然产物研究与开发, 2024, 36(4): 694-716.
[10]	张一宁, 于志琪, 屈施旭, 刘同歌, 张玉红. 火麻叶中大麻二酚提取工艺的响应面法优化及不同部位含量分布[J]. 天然产物研究与开发, 2024, 36(3): 369-376.
[11]	邵林子, 程玉娇, 黄林华, 谈安群, 王华. 超声辅助深共晶溶剂高效提取枳实辛弗林的工艺优化及提取动力学分析[J]. 天然产物研究与开发, 2024, 36(3): 499-511.
[12]	宋丽娅, 李丽华, 毕思童. 基于网络药理学、分子对接技术及实验验证研究黄芪提取物治疗缺血缺氧性脑病的机制[J]. 天然产物研究与开发, 2024, 36(3): 520-527.
[13]	张闻婷, 杨旻静, 梅瑜, 王继华. 金线莲苷的研究现状[J]. 天然产物研究与开发, 2024, 36(2): 348-356.
[14]	吕福杰, 范在举, 李琦, 邓爱霞. 金果榄二萜类成分及其抗炎活性研究[J]. 天然产物研究与开发, 2024, 36(10): 1707-1713.
[15]	丁芙蓉, 贾小演, 吴相欢, 吴霞, 杨念, 刘雄利, 田民义. 黄姜花花的化学成分、抗氧化、酶抑制活性及抗炎作用研究[J]. 天然产物研究与开发, 2024, 36(1): 13-25.