天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (4): 591-595.

• 研究论文 •    下一篇

海洋链霉菌Streptomyces lusitanus SCSIO 32中芳酰胺类代谢产物的研究

任香梅1,2，黄洪波1，刘 静1,2，张 云1,2，马俊英1，王 博1，张长生1，鞠建华1*   

  1. 1中国科学院海洋生物资源可持续利用重点实验室，中国科学院海洋微生物中心，广东省海洋药物重点实验室，中国科学院南海海洋研究所，广州510301；2中国科学院研究生院，北京 100049
  • 收稿日期:2011-02-28 出版日期:2011-08-25 发布日期:2011-09-13
  • 基金资助:

    中国科学院知识创新工程重要方向项目（KSCX2-YW-G-065, KZCX2-YW-JC202, KSCX2-YW-G-073, KZCX2-EW-G-12）；中国科学院南海海洋所领域前沿项目（LYQY200805）

Aromatic Amide Metabolites from the Marine-derived Streptomyces lusitanus SCSIO 32

REN Xiang-mei1,2, HUANG Hong-bo1, LIU Jing1,2, ZHANG Yun1,2, MA Jun-ying1, WANG Bo1, ZHANG Chang-sheng1, JU Jian-hua1*   

  1. 1The Chinese Academy of Sciences Key Laboratory of Marine Bio-resourses Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Guangzhou 510301, China; 2Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-02-28 Online:2011-08-25 Published:2011-09-13

摘要： 采用硅胶柱色谱和半制备反相高效液相色谱分离方法，对南海海洋放线菌Streptomyces lusitanus SCSIO 32的次级代谢产物进行了研究，分离得到四个酰胺类化合物，经MS、1H和13C NMR波谱分析鉴定为二甲基甲苯2,4-二氨基甲酸甲酯（1），甲苯2,4-二氨基甲酸乙酯（2），甲苯2,6-二氨基甲酸甲酯（3）和甲苯2,6-二氨基甲酸乙酯（4）。运用X-单晶衍射确定了1的结构式。其中化合物2和4是首次从自然界中分离得到。采用16S分子生物学方法鉴定该菌株为链霉菌属放线菌。

关键词: 海洋放线菌, 链霉菌属, 酰胺, 次级代谢产物

Abstract: Four aromatic amide metabolites, toluene-2,4-dicarbamic acid dimethyl ester (1), toluene-2,4-dicarbamic acid diethyl ester (2), toluene-2,6-dicarbamic acid dimethyl ester (3) and toluene-2,6-dicarbamic acid diethyl ester (4) were isolated from the marine-derived actinomycetes Streptomyces lusitanus SCSIO 32 from the South China Sea with silica gel column chromatography and revered phase semipreparative-HPLC. Their structures were identified by MS, 1H and 13C NMR spectral data analyses. The structure of 1 was confirmed by single crystal X-ray diffraction. Compounds 2 and 4 were obtained from natural sources for the first time. The strain was identified as a member of Streptomyces genus by analysis of its 16S rRNA gene sequence.

Key words: marine actinomycetes, Streptomyces lusitanus, aromatic amide, secondary metabolites

中图分类号: 

Q939.13 R931.77

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