天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (4): 620-624.

• 研究论文 • 上一篇    下一篇

侧柏叶精油的抗细菌和抗氧化活性(英文)

周雨凡1,2,王蓟花2,刘志龙2,王 琦2,贺 伟1,周立刚2*   

  1. 1北京林业大学林学院,北京100083;2中国农业大学农学与生物技术学院,北京100193
  • 出版日期:2011-08-25 发布日期:2011-09-13

Antibacterial and Antioxidant Activities of the Leaf Essential Oil of Platycladus orientalis

ZHOU Yu-fan1,2, WANG Ji-hua2, LIU Zhi-long2, WANG Qi2, HE Wei1, ZHOU Li-gang2*   

  1. 1College of Forestry, Beijing Forestry University, Beijing 10083, China; 2College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
  • Online:2011-08-25 Published:2011-09-13
  • Supported by:

    This work was co-financed by the grants from the Commonweal Fund of China Agriculture (200903052), and the National Key Technology R&D Program of China (2008BADA5B03).

摘要: 采用水蒸气蒸馏法提取侧柏叶精油,通过GC和GC-MS分析精油的组成。从侧柏叶精油中鉴定出29种成分,主要为单萜和倍半萜,含量较高的化合物有:8-丙氧基-柏木烷(15.41%)、松油烯-4-醇(12.98%)、α-蒎烯(9.85%)、桧烯(9.92%)和3-蒈烯(6.77%)。侧柏叶精油具有广谱的抗细菌活性,对供试细菌的最低杀菌浓度(MBC)在0.1 mg/mL和1.0 mg/mL之间,最低抑菌浓度(MIC)在0.063 mg/mL 和 0.90 mg/mL之间,半抑制浓度(IC50)在0.044 mg/mL和0.763 mg/mL之间。侧柏叶精油能有效抑制菲啰嗪与Fe2+的螯合(IC50值为0.409 mg/mL)和β-胡萝卜素-亚油酸的氧化(IC50值为0.526 mg/mL)。

关键词: 侧柏, 精油, 化学组成, 抗细菌活性, 抗氧化活性

Abstract: The leaf essential oil of Platycladus orientalis obtained by hydro-distillation was investigated by GC and GC-MS. 8-Propoxy cedrane (15.41%), terpinen-4-ol (12.98%), α-pinene (9.85%), sabenene (9.92%), and 3-carene (6.77%) were the major compounds of the 29 identified components which accounted for 96.64% of the total oil of P. orientalis. The oil was rich in monoterpenoids and sesquiterpenoids. It was assayed to have the broad spectral antibacterial activity with the MBC values ranged from 0.10 mg/mL to 1.0 mg/mL, the MIC values from 0.063 mg/mL to 0.90 mg/mL, and the IC50 values from 0.044 mg/mL to 0.763 mg/mL. The essential oil was subjected to the screening for the antioxidant activity by ferrozine-Fe2+ complex formation and β-carotene bleaching inhibition assays. It inhibited both the formation of ferrozine-Fe2+ complex and β-carotene-linoleic acid oxidation in a dose dependent manner with the IC50 values of 0.409 mg/mL and 0.526 mg/mL, respectively.

Key words: Platycladus orientalis, essential oil, chemical composition, antibacterial activity, antioxidant activity

中图分类号: 

Q946.8 R284.1