豆皮水溶性多糖组分SHP-3的物化性质研究

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (1): 76-81.

豆皮水溶性多糖组分SHP-3的物化性质研究

赖富饶¹，吴晖^1*，李晓凤¹，任尧¹，吴磊燕^1,2，温其标¹

¹华南理工大学轻工与食品学院，广州 510640；²江西农业大学食品科学与工程学院，南昌 330045

收稿日期:2009-05-04 发布日期:2011-07-20
通讯作者: fehwu@scut.edu.cn

Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull

LAI Fu-rao¹, WU Hui^1*, LI Xiao-feng¹, REN Yao¹, WU Lei-yan^1,2, WEN Qi-biao¹

¹College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; ²College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China

Received:2009-05-04 Published:2011-07-20
Supported by:
This project was supported by Program for New Century Excellent Talents in University (NCET-06-0746).

摘要/Abstract

摘要：

对豆皮采用热水浸提得到豆皮水溶性多糖，通过DEAE-cellelose离子交换柱洗脱，得到3个组分。本文主要研究了采用0.3 M NaOH溶液洗脱纯化得到的组分SHP-3的物化性质。对SHP-3进行凝胶渗透色谱、气相色谱分析、紫外光谱及高碘酸氧化-Smith降解分析，结果表明：SHP-3的分子量为45554，其糖醛酸含量为26.71%（wt. %），单糖组成摩尔比为Rah: Fuc: Ara: Xyl: Man: Gal: Glu = 3.55: 0.44: 11.58: 1: 7.45: 5.12: 1.12，紫外光谱在260~280 nm没有吸收峰，表明没有蛋白质或核酸等物质；高碘酸氧化-Smith降解结果表明SHP-3的连接结构以为(1→4)糖苷键为主，其摩尔比例占68.9%，(1→2)糖苷键占11.4%，(1→6)糖苷键占19.7%。

关键词: 豆皮, 水溶性多糖, 纯化, 单糖组成, 气相色谱

Abstract:

The water-soluble polysaccharide of soybean hull (SHP) was extracted by hot water and three fractions were obtained by using DEAE-cellulose anion exchange column. The physicochemical properties of the purified fractions SHP-3, eluted with 0.3 M NaOH, were investigated. Gel permeation chromatography showed that the molecule weight of SHP-3 was 45554. GC analysis of the monosaccharide composition showed that the molar ratio of Rah: Fuc: Ara: Xyl: Man: Gal: Glu was 3.55:0.44:11.58:1:7.45:5.12:1.12 and the content of galacturonic acid was 26.71% (wt.%). The ultraviolet spectra indicated that there was no presence of protein in SHP-3. The molar percentages of (1→2) glycosidic linkage, (1→4) glycosidic linkage and (1→6) glycosidic linkage were 11.4%, 68.9% and 19.7%, respectively.

Key words: soybean hull, water-soluble polysaccharide, purification, monosaccharide composition, GC

赖富饶，吴晖，李晓凤，任尧，吴磊燕，温其标. 豆皮水溶性多糖组分SHP-3的物化性质研究[J]. 天然产物研究与开发, 2011, 23(1): 76-81.

LAI Fu-Rao, WU Hui, LI Xiao-Feng, REN Yao, WU Lei-Yan, WEN Qi-Biao. Physicochemical Properties of a Water-soluble Polysaccharide Fraction SHP-3 Purified from Soybean Hull[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 76-81.

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