HPLC法测定槟榔中的多酚类物质

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (1): 101-104.

HPLC法测定槟榔中的多酚类物质

王明月^1,2*，罗金辉²，李建国²

¹中国热带农业科学院热带生物技术研究所；²中国热带农业科学院分析测试中心，海口 571101

收稿日期:2009-06-03 发布日期:2011-07-20
通讯作者: hkwmy0815@163.com
基金资助:
2008年中央级公益性科研院所基本科研业务费（ITBBKF083）

Determination of Polyphenols in Areca catechu by HPLC

WANG Ming-yue^1,2*, LUO Jing-hui², LI Jian-guo²

¹Analysis and testing centre, Chinese Academic of Tropical Agricultural Sciences; ²Biotechnology Research Institute of Tropical, Chinese Academic of Tropical Agricultural Sciences, Hainan 571101, China

Received:2009-06-03 Published:2011-07-20

摘要/Abstract

摘要：

采用高效液相色谱法对槟榔中的多酚类物质进行分析。用甲醇提取槟榔中的多酚类物质，并依次用石油醚、乙酸乙酯、正丁醇萃取，萃取物经抽真空浓缩，流动相定容，高效液相色谱法测定。分析结果表明：槟榔幼果较槟榔成熟果中所含的多酚种类和数量少，槟榔成熟果中果仁的多酚种类和数量都远较皮中多；槟榔的甲醇提取物乙酸乙酯萃取部分中所含的多酚种类和数量相比石油醚和正丁醇萃取部分都是最多的；槟榔仁的多酚中含量最多的是儿茶素，含量为1610mg/kg，其次是单宁酸622 mg/kg，表没食子儿茶素228mg/kg，表儿茶素164mg/kg，表没食子儿茶素没食子酸酯72mg/kg，没食子酸13.6mg/kg
。而没食子儿茶素、绿原酸、和没食子儿茶素没食子酸酯没有检测到。

关键词: HPLC法, 槟榔, 多酚类物质

Abstract:

HPLC was used to determine polyphenols in Areca catechu. Polyphenols were extracted from betel nut with methanol, which were extracted with petroleum ether, ethyl acetate, and n-butanol, successively. Then, the extracts were concentrated and determined by HPLC after making constant volume using the mobile phase. The results showed that the kinds and quantities of polyphenols in mature young betel nut were fewer than in mature fruit, while those in nuts of mature fruit were more than in the skin. The kinds and quantities of polyphenols in the methanol extract were the most. The content of catechin was the highest reaching 1610mg/kg in the polyphenols, tannic acid (622 mg/kg), epigallocatechin (228 mg/kg), epicatechin (164 mg/kg), epigallocatechin gallate (72 mg/kg), and gallic acid (13.6 mg/kg) were followed. However, catechin gallate, chlorogenic acid, and gallocatechin gallate were not detected.

Key words: HPLC, Areca catechu, polyphenols

王明月，罗金辉，李建国. HPLC法测定槟榔中的多酚类物质[J]. 天然产物研究与开发, 2011, 23(1): 101-104.

WANG Ming-Yue, LUO Jin-Hui, LI Jian-Guo. Determination of Polyphenols in Areca catechu by HPLC[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(1): 101-104.

参考文献

1 Zhang CJ(张春江),Lv fj(吕飞杰),Tao HT(陶海腾).The research progress of active ingredient in betel nut and its functional role.Food Nutr China(中国食物与营养),2008,6:50-53.
2 Xiao PG(肖培根).Modern Chinese Material Medica(新编中药志).Beijing:Chemical Industry Press,2002:643-647.
3 Huang BB(黄冰冰),Fan MW(樊明文),Yang XL(杨祥良),et al.The impact of Chinese herbal medicine on the growth of periodontal bacteria.J Four Mil Med Univ(第四军医大学学报),2003,24:424-426.
4 Xiao Y(肖悦),Liu TJ(刘天佳),Huang ZW(黄正蔚),et al.The impact of 5 kinds of natural drugs on Streptococcus mutans in saliva of acquired membrane adhesion.J Sichuan Univ,Med Ed (四川大学学报，医学版),2004,35:687-689.
5 Lee KK,Cho JJ,Choi JD.Anti-elastase and anti-hyalunon idase of phenolic substance from areca catechu as new anti-ageing agent.Int J Cosmet Sic,2001,23:341-346.
6 Jeon SM,Kim HS,Lee TG,et al.Lower absorption of cholesteryloleate in rats supplem ented with areca catechul extract.Ann Nutr Metab,2000,44:170-176.
7 Byun SJ,Kim HS,Jeon SM,et al.Supplem entation of areca catechu L.ettract alters triglyceride absorption and cholesterolm etabolism in rats. Ann Nutr Metab,2001,45:279-284.
8 Guota PC,Wamaku lasuriya S.Global epidem iology of areca nut usage.Addict Biol,2002,(7):77-83.
9 Yu WJ(愈文杰).Observation of 21 kinds of Chinese medicine on the role of fibrinolysis in vitro observation.J Integr Tradit West Med (中西医结合杂志),1986,6:484.
10 Ou XC(欧兴长),Ding JX(丁家欣),Zhang L(张玲).Journal of Integrated Traditional and Western Medicine . Chinese Tradic Herb Med (中草药),1987,18(40):21.
11 Wang JK(王进昆),Sun LX(孙路西).Betel quid in the analysis of phenolic compounds.Chin Agric Chem Soc (中国农业化学会志),1993,31:623-632.
12 Zhang CJ(张春江),Lv FJ(吕飞杰),Tai JX(台建祥),et al.Fruit and nut products in the total phenol and tannin content determined.Food Res Dev (食品研究与开发),2008,6:119-121.
13 Chen L(陈磊),Yang JL(杨建荣),Huang XS(黄雪松).Determination of 6 kinds of polyphenols in Fuji apple pomace by HPLC.Food Ferment Ind(食品与发酵工业),2008,34(8):158-161.
14 Sun HF(孙海峰),Lv HT(吕海涛),Zhou SS(周莎莎),et al.Determination of polyphenols in apple juice concentrate by HPLC.Food Sci (食品科学),2008,29:314-317。
15 Zhang JF(张晋芬),Yuan B(袁冰),Leng P(冷平),et al.Determination of 8 kinds of Polyphenols in pears, apples and bananas with the microwave extraction and high-performance liquid chromatography.J Anal Test (分析测试学报),2008,27:1371-1374.

[1]	策力木格, 许良. 槟榔化学成分、药理、毒性研究进展及质量标志物预测分析[J]. 天然产物研究与开发, 2023, 35(8): 1431-1441.
[2]	牛江秀1，陈洪1，张岩松1,2，王素军1，押辉远1，许启泰1,2*. 槟榔深加工产品开发研究进展[J]. 天然产物研究与开发, 2017, 29(增刊1): 221-224.
[3]	刘永芹1，邸迎彤2，王跃虎2，胡旭佳1*. 马槟榔种仁化学成分研究[J]. 天然产物研究与开发, 2017, 29(3): 415-418.
[4]	吴娇1，于海川2*，赵杰1，王琳3，侯贝贝1，石梦斐，田坤1. 槟榔提取物及其不同极性部位对H₂O₂损伤的SH-SY5Y细胞的保护作用[J]. 天然产物研究与开发, 2015, 27(Suppl): 109-112.
[5]	谭友莉，马云桐*，黄凤，文静，余志芳，欧泉. 响应面法对青稞红曲中两种Monacolin K的提取工艺优化[J]. 天然产物研究与开发, 2015, 27(11): 1930-1937.
[6]	胡祖艳1,2，范青飞1，冯峰3，杨俊林1,2，宋启示1*. 槟榔青茎皮的化学成分研究[J]. 天然产物研究与开发, 2014, 26(Suppl2): 190-193.
[7]	廖金华1，胡旭佳1，苑春茂2，何红平2，邸迎彤2. 马槟榔果实的化学成分研究[J]. 天然产物研究与开发, 2014, 26(11): 1780-1784.