紫球藻及其胞外多糖对小鼠免疫功能的调节作用

摘要/Abstract

摘要：

探讨紫球藻及其胞外多糖对免疫低下小鼠免疫功能的调节作用，用环磷酰胺（CY）建立昆明种小鼠免疫功能低下的实验模型。小鼠随机均分6组，其中2组每天分别给予紫球藻藻粉1200 mg/kg、600 mg/kg灌胃，2组给予紫球藻胞外多糖300 mg/kg、150 mg/kg灌胃，正常对照组及CY组用等容积生理盐水灌胃，连续14 d。实验结果表明紫球藻及其胞外多糖均可显著提高免疫抑制小鼠的脾指数、胸腺指数、碳廓清能力、单核细胞吞噬功能，并可对抗环磷酰胺引起的外周血白细胞数目下降，因此紫球藻藻粉及其胞外多糖对小鼠免疫功能具有一定的正向调节作用。毒性实验表明，小鼠腹腔注射300 mg/kg、150 mg/kg紫球藻胞外多糖溶液和分别给予紫球藻藻粉1200 mg/kg、600 mg/kg灌胃，小鼠未出现死亡，也未有明显毒性反应出现的一些指标变化，说明紫球藻藻粉及胞外多糖的安全性较大。

关键词: 紫球藻, 胞外多糖, 免疫, 毒性实验

Abstract:

The immunomodulations of Porphyridium cruentum and its exopolysaccharide were investigated by establishment of cyclophosphamide (CY) induced immunosuppressive mice model. The mice randomly divided into six groups. Among them，two groups were given 1200 mg/kg and 600 mg/kg the P. cruentum powder by gastric perfusion respectively，and the other two groups were given 300 mg/kg and 150 mg/kg the exopolysaccharide P. cruentum by gastric perfusion respectively. The treatment carried out 14 days. The results showed that the P. cruentum powder and the exopolysaccharides could obviously increase the levels of index of spleen and thymus，the clearance of charcoal particle，and the phagocytic function of mononuclear-macrophage in the mice，while decrease the number of peripheral blood leucocyte. So Porphyridium cruentum and its exopolysaccharide had the abilities to up-regulate the immunity in vivo. In the toxicity test，the mice randomly divided into five groups. Among them，two groups were injected 300 mg/kg and 150 mg/kg the exopolysaccharide respectively，and the other two groups were given 1200 mg/kg and 600 mg/kg the powder of P. cruentum by gastric perfusion respectively. As the result，no mouse died which indicated Porphyridium cruentum and its exopolysaccharide were safety.

Key words: Porphyridium cruentum, exopolysaccharides, immunomodulation, toxicity test

林丽琴，刘丽平，黄键，陈必链. 紫球藻及其胞外多糖对小鼠免疫功能的调节作用[J]. 天然产物研究与开发, 2011, 23(2): 236-239.

LIN Li-Qin, LIU Li-Ping, HUANG Jian, CHEN Bi-Lian. Immunomodulation of Porphyridium cruentum and Its Exopolysaccharides in Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 236-239.

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