仙人掌提取物对浅Ⅱ度烫伤小鼠VEGF及FGF-2表达的影响

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (2): 246-249.

仙人掌提取物对浅Ⅱ度烫伤小鼠VEGF及FGF-2表达的影响

陈世兴，王亮，杨哲，颜栋，辛毅，张翠丽^*

大连医科大学生物技术系，大连 116044

收稿日期:2010-04-27 出版日期:2011-04-25 发布日期:2011-10-14
通讯作者: biot-zhangcl@sina.com
基金资助:
大连医科大学大学生科技创新活动基金资助项目

Effect of Cactus Extracts on the Expression of VEGF and FGF-2 in Rats with Superficial Second-degree Burns

CHEN Shi-xing, WANG Liang, YANG Zhe, YAN Dong, XIN Yi, ZHANG Cui-li^*

Biotechnology Department of Dalian Medical University, Dalian, 116044

Received:2010-04-27 Online:2011-04-25 Published:2011-10-14

摘要/Abstract

摘要：

为探讨野生仙人掌和食用仙人掌提取物对浅Ⅱ度烫伤小鼠内源性血管内皮生长因子（VEGF）及碱性成纤维细胞生长因子（FGF-2）表达的影响，建立小鼠浅Ⅱ度烫伤模型。野生、食用仙人掌提取物组（浓度均为12.5 mg/ml），药物组（京万红），烫伤组（生理盐水），每天2次涂药并观察创面，分别在3 d和7 d各组取5只小鼠处死，取烫伤组织提取蛋白并检测VEGF、FGF-2的表达状况。仙人掌组中VEGF及FGF-2的表达量均高于烫伤组，且两种仙人掌组VEGF及FGF-2的表达量有差异。即仙人掌提取物能提高VEGF及FGF-2的表达量并能促进创面的修复。

关键词: 仙人掌提取物, 浅Ⅱ烫伤, VEGF, FGF-2

Abstract:

In order to determine the effects of wild cactus extracts and edible cactus extracts on the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor 2 (FGF-2) at the superficial second-degree burns，the superficial second-degree burned mice were divided into four groups randomly; wild cactus extracts treated group，edible cactus extracts treated group，drag treated group，and burned group (the wound without treatment). Expression of growth factor of VEGF and FGF-2 were measured by western-blotting. Expressions of VEGF and FGF-2 were higher in wild cactus extracts treated group and edible cactus extracts treated group than those in other groups，but they were different in wild cactus extracts treated group and edible cactus extracts treated group. In conclusion，both wild cactus extracts and edible cactus extracts enhanced the expression of VEGF and FGF and promoted wound healing.

Key words: cactus extracts, superficial second-degree burns, VEGF, FGF-2

陈世兴，王亮，杨哲，颜栋，辛毅，张翠丽. 仙人掌提取物对浅Ⅱ度烫伤小鼠VEGF及FGF-2表达的影响[J]. 天然产物研究与开发, 2011, 23(2): 246-249.

CHEN Shi-Xing, WANG Liang, YANG Zhe, YAN Dong, XIN Yi, ZHANG Cui-Li. Effect of Cactus Extracts on the Expression of VEGF and FGF-2 in Rats with Superficial Second-degree Burns[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 246-249.

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