胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件优化

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (2): 295-298.

胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件优化

梅林，王志耕^*，王子龙，薛秀恒

安徽农业大学茶与食品科技学院，合肥 230036

收稿日期:2010-12-10 出版日期:2011-04-25 发布日期:2011-10-14
通讯作者: wzhg56@yahoo.com.cn
基金资助:
科技部科技人员服务企业行动项目（2009JGC30016）

Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide

MEI Lin, WANG Zhi-geng^*, WANG Zi-long, XUE Xiu-heng

College of Tea & Food Science and Technology of Anhui Agricultural University, Hefei 230036

Received:2010-12-10 Online:2011-04-25 Published:2011-10-14

摘要/Abstract

摘要：

采用三因素二次通用旋转设计和体外检测法，对胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件进行优化。结果表明，底物浓度（X₁）、温度（X₂）、酶与底物的质量比（X₃）对ACE抑制率的影响回归方程为：Y=50.62–2.33X₁–1.97X₂+5.81X₃–3.36X₂X₃–6.56X₂₂–1.96X₃₂，胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的最优水解条件为：底物质量浓度为60 g/L，水解温度30 ℃，酶与底物的质量比为5.5%，水解时间6 h，水解产物对ACE抑制活性最大抑制率为53.86%。

关键词: β-乳球蛋白, 胰蛋白酶, ACE

Abstract:

Three-factor quadratic orthogonal rotation design and in vitro assay was adopted to optimize the hydrolysis conditions of β-lactoglobulin with trypsin for obtaining the Angiotensin-I-converting enzyme (ACE) inhibitory peptide. The results showed that the regression equation of the impacts of the substrate concentration (X₁)，temperature (X₂)，enzyme，and substrate mass ratio (X₃) on the ACE inhibition rate was Y = 50.62–2.33X₁–1.97X₂+5.81 X₃–3. 36X₂X₃–6.56X₂₂–1.96X₃₂. The optimum conditions were as following: when substrate concentration was at 60 g/L，the hydrolysis temperature at 30 ℃，enzyme and substrate mass ratio at 5.5%，the hydrolysis time in 6 h，the hydrolysis product of ACE inhibitory activity was on the maximal inhibition rate of 53.86%.

Key words: β-lactoglobulin, trypsin, ACE

梅林，王志耕，王子龙，薛秀恒. 胰蛋白酶水解β-乳球蛋白获得ACE抑制肽的条件优化[J]. 天然产物研究与开发, 2011, 23(2): 295-298.

MEI Lin, WANG Zhi-Geng, WANG Zi-Long, XUE Xiu-Heng. Optimizing Hydrolysis Conditions of β-Lactoglobulin with Trypsin to Obtain the ACE Inhibitory Peptide[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(2): 295-298.

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