构树皮中具有α-葡萄糖苷酶抑制活性的成分

天然产物研究与开发 ›› 2010, Vol. 23 ›› Issue (6): 934-936.

构树皮中具有α-葡萄糖苷酶抑制活性的成分

鲍玲²，崔文峰²，郁建平²，喻凯³，王明奎^1*

¹中国科学院成都生物研究所，成都 610041；²贵州大学生命科学学院，贵阳 5500252；³西南交通大学生物工程学院，成都 610031

收稿日期:2009-02-11 出版日期:2011-01-10 发布日期:2011-10-19
通讯作者: wangmk@cib.ac.cn
基金资助:
四川省应用基础研究项目 (2007J13-059)

Chemical Constituents with α-Glycosidase Inhibiting Activity from the Bark of Broussonetia papyrifera

BAO Ling², CUI Wen-feng², YU Jian-ping², YU Kai³, WANG Ming-kui^1*

¹Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041;²The College of Biology in Guizhou University, Gui Yang 5500250;³School of Life Science & Engineering, Southwest Jiaotong University, Chengdu 610013

Received:2009-02-11 Online:2011-01-10 Published:2011-10-19

摘要/Abstract

摘要：

本文报道构树皮中?-葡萄糖苷酶抑制剂的分离鉴定。构树皮的乙醇浸膏通过溶剂萃取以及各种柱层析方法分离纯化得到6个化合物，通过波谱学方法鉴定为：broussonetine A (1)，broussonetinine A (2)，broussonetinine B (3)，黑立脂素苷( liriodendrin) (4)，butyrospermyl acetate (5)以及胡萝卜苷 (6)。所有化合物均首次从构树中分离得到，其中1~3具有较强抑制?-葡萄糖苷酶的活性，IC₅₀有分别为0.530、0.445和0.460 mg/mL。

关键词: 构树, broussonetine A, broussonetinine A, broussonetinine B, &alpha, -葡萄糖苷酶抑制剂

Abstract:

The α-glycosidase inhibitors were isolated and identified from the bark of Broussonetia papyrifera. By solvent extraction and column chromatography, six compounds were isolated from ethanolic extracts of this plant for the first time and identified as broussonetine A (1), broussonetinine A (2), broussonetinine B (3), liriodendrin (4), butyrospermyl acetate (5), and daucosterol (6) by spectral evidence. Activity assay indicated that compounds 1-3 had strong glycosidase inhibiting activities with IC₅₀ value of 0.530, 0.445, and 0.460 mg/ml, respectively.

Key words: Broussonetia papyrifera, broussonetine A, broussonetinine A, broussonetinine B, glycosidase inhibitor

鲍玲，崔文峰，郁建平，喻凯，王明奎. 构树皮中具有α-葡萄糖苷酶抑制活性的成分[J]. 天然产物研究与开发, 2010, 23(6): 934-936.

BAO Ling, CUI Wen-Feng, YU Jian-Ping, YU Kai, WANG Ming-Kui. Chemical Constituents with α-Glycosidase Inhibiting Activity from the Bark of Broussonetia papyrifera[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2010, 23(6): 934-936.

参考文献

1 China Pharmaceutical University(中国药科大学).Collection Words of Chinese Materia Medica (中药辞海),Vol.2. Beijing:China Medico-Pharmacological Science and Technology Publishing House,1996.695.
2Shibano M,Kitagawa S,Kusano G. Studies on the constituents of Broussonetia species.I.Two new pyrrolidine alkaloids, broussonetines C and D,as ?-galactosidase and ?-mannosidase inhibitors from Broussonetia kazinoki Sieb.Chem Pharm Bull,1997,45:505-508.
3 Shibano M,Kitagawa S,Nakamura S,et al.Studies on t he constituent s of Broussonetia species.Ⅱ.Six new pyrrolidine alkaloids, broussonetine A, B, E,F and broussonetinine A and B, as inhibitors of glycosidases from Broussonetia kazinoki Sieb.Chem Pharm Bull,1997,45:700-705.
4 Shibano M,Nakamura S,Akazawa N,et al.Studies on the constituents of Broussonetia species.III.Two new pyrrolidine alkaloids, broussonetines G and H,as inhibitors of glycosidase, from Broussonetia kazinoki Sieb.Chem Pharm Bull,1998, 46: 1048-1050.
5 Shibano M,Nakamura S,Kubori M,et al.Studies on the constituents of Broussonetia species.IV.Two new pyrrolidinyl piperidine alkaloids, roussonetines I and J,from Broussonetia kazinoki Sieb.Chem Pharm Bull,1998,46:1416-1420.
6 Shibano M,Nakamura S,Motoya N,et al.Studies on the constituents of Broussonetia species.V.Two new pyrrolidine alkaloids,broussonetines K and L,as inhibitors of glycosidase, from Broussonetia kazinoki Sieb.Chem Pharm Bull,1999, 47:472-476.
7 Shibano M, Tsukamoto D,Kusano G.A new pyrrolizidine alkaloid,broussonetine N,as an inhibitor of glycosidase,from Broussonetia kazinoki Sieb.and absolute stereost ructures of broussonetines A and B.Chem Pharm Bull,1999,47:907-908.
8 Shibano M,Tsukamoto D,Fujimoto R,et al.Studies on t he constituent s of Broussonetia species.VII.Four new pyrrolidine alkaloids,broussonetines M,O,P,and Q,as inhibitors of glycosidase, from Broussonetia kazinoki Sieb.Chem Pharm Bull,2000,48:1281-1285.
9 Tsukamoto D,Shibano M,Okamoto R,et al.Studies on the constituents of Broussonetia species Ⅷ. Four new pyrrolidine alkaloids, broussonetines R, S,T,and V and a new pyrroline alkaloid, broussonetine U,from Broussonetia kazinoki Sieb. Chem Pharm Bull,2001,49:492-496.
10 Tsukamoto D,Shibano M,Kusano G.Studies on the constituents of Broussonetia species Ⅹ.Six new alkaloids from Broussonetia kazinoki Sieb.Chem Pharm Bull,2001,49:1487-1491.
11 Lee DH, Kinghorn AD.Bioactive compounds from the genus Broussonetia.Studies in Natural Products chemistry, 2003,28:3-33.
12 Li ZQ (李子清),Yu k (喻凯),Zhao HY (赵焕英).The development and application of a screening model for intestine α-Glycosidase inhibitor.J Southwest Jiaotong Univ (西南交通大学学报).2006, 41 (增刊):152-154.
13 Jolad SD,Hoffmann JJ,Cole JR,et al.Cytotoxic agent from Penstemon deustus ( Scrophulariaceae ):isolation and stereochemistry of liriodendrin, a symmetrically substituted furofuranoid lignan diglucoside.J Org Chem,1980, 45:1327-1329.
14 Akihisa T,Qinuma H,Yasukawa K,et al.Helianol
[3, 4-seco-19 (10→9) abeo-8α, 9β, 10α-eupha-4, 24-dien-3-ol ],a novel triterpene alcohol from the tabular flowers of Helianthus annuus L. .Chem Pharm Bull,1996,44:1255-1257.
15 Heightman TD,Vasella AT.Recent insights in inhibition,structure,and mechanism of configuration-retaining glycosidases.Angew Chem,int ed,1999,38:750-770.

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