丹酚酸B对高脂日粮小鼠抗氧化与淋巴细胞亚群免疫功能调节的研究

天然产物研究与开发 ›› 2010, Vol. 23 ›› Issue (6): 965-971.

丹酚酸B对高脂日粮小鼠抗氧化与淋巴细胞亚群免疫功能调节的研究

王斌²，崔珏²，肖瀛²，施用晖^1,2，乐国伟^1,2*

¹江南大学食品科学与技术国家重点实验室，无锡 214122； ²江南大学食品学院食品营养与安全研究所，无锡 214122

收稿日期:2009-02-25 出版日期:2011-01-10 发布日期:2011-10-19
通讯作者: lgw@jiangnan.edu.cn
基金资助:
国家自然基金(30571347)；科技部十一五支撑计划项目(2006BAD27B06)

Antioxidation and Immunomodulatory Function on Lymphocyte Subsets of Salvianolic Acid B in High-fat Diet Fed Mouse

WANG Bin² , CUI Jue² , XIAO Ying² , SHI Yong-hui^1,2 , LE Guo-wei^1,2*

¹State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; ²Institute of Nutrition and Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2009-02-25 Online:2011-01-10 Published:2011-10-19

摘要/Abstract

摘要：

本文研究了丹酚酸B（Salvianolic acid B, Sal B）对饲喂高脂日粮小鼠抗氧化及淋巴细胞亚群免疫功能调节的影响。选取4 w龄雄性C57BL/6小鼠40只，随机平均分成4组：即正常对照组（正常日粮），高脂日粮组（高脂日粮），Sal B低剂量组（高脂日粮+0.002% Sal B）和Sal B高剂量组（高脂日粮+0.004% Sal B）。八周后测定血脂、全血和脾脏活性氧（reactive oxygen species, ROS）水平、抗氧化酶活性及MDA含量；采用FACSAria流式细胞仪分析小鼠外周血及脾脏淋巴细胞亚群。结果表明：Sal B可显著降低高脂日粮小鼠血脂水平，全血及脾脏ROS水平，提高抗氧化能力，提高CD4⁺/CD8⁺T细胞比例及CD19+细胞百分率（P＜0.05）。低剂量Sal B组抗氧化与免疫调节功能好于高剂量Sal B组。

关键词: 高脂日粮, 丹酚酸B, 血脂, 活性氧, 抗氧化酶, 淋巴细胞亚群

Abstract:

This article illustrates the effects of Salvianolic acid B (Sal B) on the antioxidation and immunomodulatory function of lymphocyte subsets in high-fat diet fed mouse. Forty male C57BL/6 mice were randomly assigned to four groups. The control group consumed an ordinary diet. The other three experimental groups were fed with a high-fat diet, a high-fat diet plus 0.002% Sal B, and a high-fat diet plus 0.004% Sal B, respectively. After 8 weeks, the plasma lipid status, reactive oxygen species (ROS) level, actioxidase activity and the content of MDA in blood and spleen were examined. The proportion of lymphocyte subsets were determined by FACSAria Flow Cytometer. The results indicated that supplementation of Sal B could decrease the levels of plasma lipid and ROS and increase the antioxidation index of high-fat diet mice. It could increase the ratio of CD4⁺and CD8⁺T cell as well as the ratio of CD19⁺ cell in experimental groups. The antioxidation and immunoregulation effects of low dose of Sal B were better than those of the high dose.

Key words: high-fat diet, Salvianolic acid B, plasma lipid, reactive oxygen species, antioxidase, lymphocyte subsets

王斌，崔珏，肖瀛，施用晖，乐国伟. 丹酚酸B对高脂日粮小鼠抗氧化与淋巴细胞亚群免疫功能调节的研究[J]. 天然产物研究与开发, 2010, 23(6): 965-971.

WANG Bin, CUI Jue, XIAO Ying, SHI Yong-Hui, LE Guo-Wei. Antioxidation and Immunomodulatory Function on Lymphocyte Subsets of Salvianolic Acid B in High-fat Diet Fed Mouse[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2010, 23(6): 965-971.

参考文献

1 Chen X,Zhong HY,Zeng JH,et al.The pharmacological effect of polysaccharides from Lentinus edodes on the oxidative status and expression of VCAM-1mRNA of thoracic aorta endothelial cell in high-fat-diet rats.Carbohydr Polym,2008,74:445-450.
2 Chan SHH,Tai MH,Li CY,et al.Reduction in molecular synthesis or enzyme activity of superoxide dismutases and catalase contributes to oxidative stress and neurogenic hypertension in spontaneously hypertensive rats.Free Radical Biol Med,2006,40:2028-2039.
3 Kohen R,Nyska A.Oxidation of biological systems:oxidative stress phenomena, antioxidants, redox reactions,and methods for their quantification.Toxicol Pathol,2002,30:620-650.
4 Guo ZG(郭志刚),Chen Y(陈瑗),Lu ZG(陆振刚),et al.The injury of lipid hydroperoxide and lipoperoxidatively modified LDL to immunologic function of macrophage.Chin J Microbiology and Immunology(中华微生物学和免疫学杂志),1991,11:226-229.
5 Du GH(杜冠华),Zhang JT(张均田).Water soluble active ingredients of Danshen--review on the Salvianolic acids.Basic Med Sci Clinics(基础医学与临床),2000,20:394-395.
6 Du R(杜嵘),Zhang BL(张伯礼),Gao XM(高秀梅),et al.Effects of salveanolic acid B on expression of intercellular adhesion molecule-1 on cardiac microvascular endothelial cells of hypoxia.Chin J New Drugs Clin Rem(中国新药与临床杂志),2004,23:616-618.
7 Navid E,Sharma RK,Saleh RA,et al.Utility of the nitroblue tetrazolium reduction test for assessment of reactive oxygen species production by seminal leukocytes and spermatozoa.J Androl,2003,24:862-870.
8 Andras N,Judit J,Tamas K,et al.Physico chemical modeling of the role of free radicals in photodynamic therapy.Biochem. Biophys Res Commun.1999,255:360-366.
9 Li YB,Zhu H,Trush MA.Detection of mitochondria-derived reactive oxygen species production by the chemilumigenic probes lucigenin and luminol.Biochim Biophys Acta,1999,1428:1-12.
10 Xu YJ(许雅娟),Zhao YJ(赵艳景),Hu H(胡虹).Research on the measurement of the SOD activity via pyrogallol auto-oxidation.J Southwest Univ Nat,Nat Sci(西南民族大学,自科版),2006,32:1207-1209.
11 Harada SM,Kinoshita M,Kamido H,et al.Oxidised low density lipoprotein induces apoptosis in cultured human umbilical vein endothelial cells by common and unique mechanisms.Biol Chem,1998,273:9681-9687.
12 Wang XB(王晓斌),Yu F(于锋),Liu F(刘飞).Protective effect of salvianolicacid B on oxidative injury in HUVECs and its possible mechanism.J Southeast Univ, Med Sci (东南大学学报,医学版),2008,27:42-46.
13 Dandona P,Aljada A,Chaudhuri A,et al.Metabolic syndrome:a comprehensive perspective based on interactions between obesity,diabetes,and inflammation.Circulation,2005,111:1448-1454.
14 Suematsu M,Suzuki H,Delano FA,et al.The inflammatory aspect of the microcirculation in hypertension:oxidative stress, leukocytes/endothelial interaction,apoptosis.Microcirculation,2002,9:259-276.
15 Racanelli V,Rehermann B.The liver as an immunological organ.Hepatology,2006,43:S54-S62.

[1]	周喆, 胡诗安, 胡雨瞳, 谭洋, 裴刚, 李玲. 丹酚酸B对肝损伤小鼠肠道菌群和短链脂肪酸代谢的影响[J]. 天然产物研究与开发, 2024, 36(5): 748-754.
[2]	徐元庆, 张静, 王哲奇, 红雷. 岩藻多糖对大鼠氧化应激的缓解作用研究[J]. 天然产物研究与开发, 2024, 36(4): 653-659.
[3]	王庆婷, 耿晓桐, 李雅静, 张娟, 刘庆普, 龚海燕, 雷敬卫, 谢彩侠. 盾叶薯蓣响应低磷胁迫的生理代谢及基因表达特征分析[J]. 天然产物研究与开发, 2023, 35(9): 1569-1582.
[4]	边惠琴, 武晓玉, 夏鹏飞, 段文达, 杨飞霞, 李娇娇, 赵磊. 化学修饰L02肝细胞膜生物亲和材料快速筛选大黄降血脂活性成分[J]. 天然产物研究与开发, 2022, 34(3): 375-382.
[5]	郝甜甜, 陈艳阳, 苗佳琪, 牛晨, 梁佳, 许永华. 茉莉酸甲酯和水杨酸对西洋参愈伤组织生长和皂苷形成的影响[J]. 天然产物研究与开发, 2022, 34(12): 2063-2068.
[6]	杜世龙, 宁秀梅, 何苗, 李翡翡, 张会, 丁兰. Leukamenin E诱导人宫颈癌HeLa细胞胞质骨架重排和迁移抑制的研究[J]. 天然产物研究与开发, 2021, 33(7): 1186-1197.
[7]	罗杨, 李娟, 陈岗, 吴振, 詹永, 廖霞, 杨勇, . 壳聚糖生姜精油涂膜对鲜银耳活性氧代谢和组织超微结构的影响[J]. 天然产物研究与开发, 2020, 32(8): 1405-1412.
[8]	刘国安, 靳亚东, 冉苗苗, 李贵琛, 杨麒励, 丁兰 . 姜黄素通过促进ROS产生诱导人脑胶质瘤细胞凋亡[J]. 天然产物研究与开发, 2020, 32(4): 541-548.
[9]	摆倩文1,2，张邵博1,2，赫欣睿1,2，诺如﹒以扎﹒诺丁3,丁功涛1*. 硫酸软骨素降血脂功能的研究[J]. 天然产物研究与开发, 2019, 31(增刊2): 79-83.
[10]	宋忠兴1,2，唐志书2*，严邑萍3，刘妍如2，刘红波2，于金高2，杨莎2，张朝2. 沙棘黄酮调节大鼠血脂代谢的药效研究[J]. 天然产物研究与开发, 2019, 31(增刊1): 115-120.
[11]	李香*，汪巍，邓莹，魏学军，孙晓惠，许义红. 三颗针不同炮制、提取方法对糖尿病小鼠血糖、血脂的影响研究[J]. 天然产物研究与开发, 2019, 31(8): 1307-1316.
[12]	单梅华，董建新，刘晓光，马雅慧，王金梅，朱毓永，李洁*. 山杏叶乙酸乙酯提取物调控乳腺癌MCF7细胞增殖和凋亡的机制研究[J]. 天然产物研究与开发, 2019, 31(7): 1124-1129.
[13]	钱凯1，何潇1，宋丹丹1，谭婷1，冯育林1,2，杨世林2，温泉1 *. 利用陶瓷膜技术精制丹酚酸B及其降糖活性的研究[J]. 天然产物研究与开发, 2019, 31(12): 2163-2169.
[14]	方敬1，陈志强1,2*，王月华3，郭倩4，张江华4. 化瘀通络中药全方及拆方对DN大鼠血液相关指标及肾脏足细胞ZO-1表达的干预作用[J]. 天然产物研究与开发, 2018, 30(7): 1235-1241.
[15]	景临林，武柠子，杨颖，何蕾，贾正平，马慧萍*. 黄芩素-7-甲醚对高原缺氧小鼠脑组织保护作用研究[J]. 天然产物研究与开发, 2018, 30(6): 1054-1060.