Enterobacter cloacae Z0206细菌胞外多糖的体外抗氧化活性研究

天然产物研究与开发 ›› 2010, Vol. 23 ›› Issue (6): 1098-1102.

Enterobacter cloacae Z0206细菌胞外多糖的体外抗氧化活性研究

徐春兰，钦传光^*，牛卫宁，尚晓娅

西北工业大学生命科学院，西安 710072

收稿日期:2009-06-30 出版日期:2011-01-10 发布日期:2011-10-19
通讯作者: lx304319@163.com
基金资助:
中国博士后科学基金（20080440195）和西北工业大学科技创新基金（2008KJ02040）

Antioxidative Activity of exopolysaccharide Produced by Enterobacter cloacae Z0206

XU Chun-lan, QIN Chuan-guang^* , NIU Wei-ning, SHANG Xiao-ya

Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China

Received:2009-06-30 Online:2011-01-10 Published:2011-10-19

摘要/Abstract

摘要：

本实验旨在对Enterobacter cloacae Z0206菌进行发酵培养，以制备胞外多糖，并对其体外抗氧化活性进行初步研究。通过产多糖菌E. cloacae Z0206的深层发酵制备细菌胞外多糖，在此基础上对其清除DPPH自由基、超氧阴离子、抑制羟自由基的能力以及还原力等四个方面进行实验，评价其抗氧化活性。结果表明，深层发酵制备的E. cloacae Z0206胞外多糖产量为6.62 g/L，其在5 mg/mL时对DPPH自由基和羟自由基的清除率分别达到61.57%和40.08%。提示E. cloacae Z0206细菌胞外多糖具有显著的抗氧化能力，具有开发为抗氧化类食品或药品的潜力。

关键词: Enterobacter cloacae, 胞外多糖, 自由基, 抗氧化

Abstract:

The experiment was conducted to prepare Exopolysaccharide (EPS) from Enterobacter cloacae Z0206 strain through fermentation and to evaluate its antioxidant activity. By determining the reducing capability, the scavenging effects of EPS on 1,1-diphenyl-2-picrylhy-drazyl (DPPH) radicals, superoxide anion free radicals, and hydroxyl radicals, the antioxidant activity of EPS were evaluated. The results showed that the yield of EPS was 6.62 g/L and the scavenging effects of EPS on the DPPH free radical and the hydroxyl radicals reached 61.57% and 40.08% at concentration of 5 mg/mL, respectively. The EPS exhibited obvious antioxidant activity and was worthy of exploitation in future.

Key words: Enterobacter cloacae, exopolysaccharide, free radicals, antioxidant

徐春兰，钦传光，牛卫宁，尚晓娅. Enterobacter cloacae Z0206细菌胞外多糖的体外抗氧化活性研究[J]. 天然产物研究与开发, 2010, 23(6): 1098-1102.

XU Chun-Lan, QIN Chuan-Guang, NIU Wei-Ning, SHANG Xiao-Ya. Antioxidative Activity of exopolysaccharide Produced by Enterobacter cloacae Z0206[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2010, 23(6): 1098-1102.

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