天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (2): 219-223.doi: A

• 开发研究 • 上一篇    下一篇

香露兜叶的抗氧化活性

王 辉1,3，罗 应1,3，梅文莉1,3，谭乐和2，徐雪荣1,3，戴好富1,3*   

  1. 1中国热带农业科学院热带生物技术研究所 农业部热带作物生物技术国家重点实验室，海口 571101;2中国热带农业科学院香料饮料研究所，万宁 571533；3海口市热带天然产物研究与利用重点实验室，海口 571101
  • 收稿日期:2010-09-16 出版日期:2012-02-29 发布日期:2012-03-23

Antioxidant Properties of Pandanus amaryllifolius Leaves

WANG Hui1,3,LUO Ying1,3,MEI Wen-li1,3,TAN Le-he2，XU Xue-rong1,3,DAI Hao-fu1,3*   

  1. 1Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;2Institute of spice and Beverage Research,Chinese Academy of Agricultural Sciences,Wanning 571533,China;3Haikou key laboratory for research and development of tropical natural products,Haikou 571101,China
  • Received:2010-09-16 Online:2012-02-29 Published:2012-03-23
  • Supported by:

    This work was supported by the National Science & Technology Pillar Program of China (2007BAI27B04) and National Nonprofit Institute Research Grant of CATAS-ITBB (No.ITBBZD1041).

摘要： 本文评价了香露兜叶的乙醇提取物以及石油醚、乙酸乙酯、正丁醇和水相组分的抗氧化活性。在体外测定了提取物和组分的总抗氧化活性，二苯代苦味肼基（DPPH）自由基清除活性，超氧阴离子自由基清除活性和铁离子还原能力，并利用分光光度法测定了其总酚和总黄酮的含量。结果表明，所有的提取物和组分均表现出抗氧化和自由基清除活性。抗氧化活性的大小如下：正丁醇部分>乙酸乙酯部分>乙醇提取物>水部分>石油醚部分。总酚含量的顺序和其相似，说明提取物和组分中的酚类化合物使其具有抗氧化活性。香露兜叶提取物可能会成为有价值的天然抗氧化资源，并将在保健品和食品中得到应用。

关键词: 香露兜, 抗氧化活性, 自由基清除活性, 酚类化合物, 黄酮类化合物

Abstract: The antioxidant activities of the ethanol extract and petroleum ether-,ethyl acetate-,n-butanol-,and aqueous-soluble fractions from Pandanus amaryllifolius leaves were investigated in this study.Total antioxidant capacity,1,1-diphenyl-2-picryhydrazyl (DPPH) scavenging activities,superoxide anion radical scavenging activities,and ferric reducing/antioxidant power (FRAP) were tested in vitro.The amounts of total phenolics and total flavonoids in the extracts and fractions were also determined by spectrophotometric methods.The results showed that all the extracts and fractions exhibited antioxidant and radical-scavenging activities.The decreasing order of antioxidant and radical-scavenging activities were found to be n-butanol fraction>ethyl acetate fraction>ethanol extract>aqueous extract>petroleum ether fraction.This similar order of the amounts of total phenolics indicated that phenolics contributed to the antioxidant activities of extract and fractions.It suggests that the extract of Pandanus amaryllifolius leaves may be valuable antioxidant natural sources and applicable in both healthcare products and food industry.

Key words: Pandanus amaryllifolius, antioxidant activities, radical-scavenging activity, phenolics, flavonoids

中图分类号:  Q284.2

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