灯盏细辛提取物对2型糖尿病小鼠氧化损伤的保护作用研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (10): 1696-1699.

灯盏细辛提取物对2型糖尿病小鼠氧化损伤的保护作用研究

王建勇1，杨庆雄2，黄小燕1*

1贵州师范大学生命科学学院；2贵州师范大学化学与材料科学学院，贵阳 550001

出版日期:2014-10-30 发布日期:2014-10-31

Protective Effect of Erigeron breviscapus Extract on Oxidative Damage in Type 2 Diabetes Mice

WANG Jiang-yong1,YANG Qing-xiong2,HUANG Xiao-yan1*

1School of Life Science,Guizhou Normal University; 2School of Chemistry and Material Science,Guizhou Normal University,Guiyang 550001, China

Online:2014-10-30 Published:2014-10-31

摘要/Abstract

摘要： 探讨灯盏细辛提取物的体内抗氧化活性以及对2型糖尿病小鼠肝脏、胰腺损伤的保护作用。采用高脂高热量饲料喂养加小剂量四氧嘧啶诱导的方法建立2型糖尿病模型，用试剂盒测定并比较了试验组与模型组及正常组的血清总超氧化物歧化酶（T-SOD）活性及丙二醛（MDA）的含量，并在光镜下观察小鼠胰腺和肝脏组织的形态学改变。实验结果表明，灯盏细辛提取物能够显著提高2型糖尿病小鼠的血清T-SOD的活性（P<0.05），明显降低其血清MDA的含量（P<0.05），各组病理损伤有较显著差异，其中模型组的损伤明显高于其余各组。可见灯盏细辛提取物具有较强的体内抗氧化作用，对2型糖尿病小鼠肝脏、胰腺损伤具有一定保护作用。

关键词: 灯盏细辛提取物, 总超氧化物歧化酶, 丙二醛, 2型糖尿病小鼠

Abstract: The objective of this study was to evaluate the antioxidant activity of Erigeron breviscapus extract and the protective effect of E.breviscapus extract on pancrea and liver injuries of type 2 diabetes mice.Type 2 diabetes mice model was induced by high-glucose-high-fat diet and small dose of alloxan.The T-SOD activity and MDA concentration of all mice serum were determined by Kit.The morphological changes of pancrea and liver tissues in mice were observed under light microscope.The results showed that the extracts of E.breviscapus can significantly increase the T-SOD activities of type 2 diabetes mellitus serum (P<0.05),and obviously decrease the concentration of MDA (P<0.05).The difference between their pathological injuries was significant(P<0.05),the model group was obviously higher than those of the other groups.It indicated that the extracts of E.breviscapus had significant antioxidant effect and had some protective effects on pancrea and liver injuries of type 2 diabetes mice.

Key words: Erigeron breviscapus extract, T-SOD, MDA, type 2 diabetes mice

中图分类号:

R285.5

王建勇1，杨庆雄2，黄小燕1*. 灯盏细辛提取物对2型糖尿病小鼠氧化损伤的保护作用研究[J]. 天然产物研究与开发, 2014, 26(10): 1696-1699.

WANG Jiang-yong1,YANG Qing-xiong2,HUANG Xiao-yan1*. Protective Effect of Erigeron breviscapus Extract on Oxidative Damage in Type 2 Diabetes Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(10): 1696-1699.

[1]	尹爱武1*，高鹏飞2，党丽敏1. 马齿苋黄酮抗衰老作用研究[J]. 天然产物研究与开发, 2017, 29(6): 988-993.
[2]	黄赛金1*，尹爱武1，刘芳玉2，万新2，田润3. 马齿苋抗大鼠乙醇型胃溃疡作用研究[J]. 天然产物研究与开发, 2016, 28(5): 781-785.
[3]	胡杰1,3，侯佳1，李月婷1,3，王爱民1,2，黄勇1,3*. 灯盏甲素在正常和脑缺血再灌注损伤大鼠体内的药代动力学研究[J]. 天然产物研究与开发, 2016, 28(5): 680-684.
[4]	姚星辰，陈湘宏，段雅彬，李向阳*. 芜菁正丁醇提取物对四氧嘧啶型糖尿病小鼠血糖的影响[J]. 天然产物研究与开发, 2015, 27(4): 706-709.
[5]	郝红伟，刘敏，王唯霖，刘新愚，马蕾，赵文*. 复方中草药提取物对小鼠机体抗氧化能力的影响[J]. 天然产物研究与开发, 2014, 26(12): 1934-1938.