中国南海来源海洋链霉菌SCSIO 11863中Enterocins的分离鉴定

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (8): 1216-1220.

中国南海来源海洋链霉菌SCSIO 11863中Enterocins的分离鉴定

Kumar Saurav，张庆波，李苏梅，张文军，田新朋，李慧贤，张海波*

中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室海洋微生物研究中心广东省海洋药物重点实验室，广州 510301

出版日期:2014-08-30 发布日期:2014-11-06

Isolation and Characterisation of Enterocins from Marine Streptomyces sp.SCSIO 11863 Isolated from South China Sea

Kumar Saurav, ZHANG Qing-bo, LI Su-mei, ZHANG Wen-jun, TIAN Xin-peng, LI Hui-xian, ZHANG Hai-bo*

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,RNAM Center for Marine Microbiology,Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences,Guangzhou 510301 ,China

Online:2014-08-30 Published:2014-11-06

摘要/Abstract

摘要： 从南海底泥样品中分离到一株具有较强抗菌活性的放线菌株SCSIO 11863。表型和进化系统分析数据表明该菌属于链霉菌属并命名为Streptomyces sp.SCSIO 11863 (KC904267)。16S rDNA 序列分析表明它与白浅灰链霉菌Streptomyces albogriseolus strain ABRIINW EA1145 (GQ925802)有99%的相似性。对该菌发酵液乙酸乙酯萃取物进行活性追踪分离得到了两个结构类似化合物，分别为Enterocin (1) 和5-deoxyenterocin (2)。

关键词: 南海沉积物, 海洋放线菌, Streptomyces sp.SCSIO 11863, enterocins

Abstract: An actinomycetes strain SCSIO 11863 with strong antibacterial activity was isolated from marine sediment of the South China Sea.Based on the phenotypic and phylogenetic analysis,the strain was identified as a member of Streptomyces and designated as Streptomyces sp.SCSIO 11863 (KC904267).16S rDNA sequence showed 99% similarity with Streptomyces albogriseolus strain ABRIINW EA1145 (GQ925802).Bioactivity guided extraction with ethyl acetate resulted in the isolation of two analogue compounds.Structures were elucidated with detailed analyses of NMR and MS spectra and identified as Enterocin (1) and 5-deoxyenterocin (2).

Key words: marine sediments of South China Sea, marine actinomycetes, Streptomyces sp.SCSIO 11863, enterocins

中图分类号:

Q936 R284.1

Kumar Saurav，张庆波，李苏梅，张文军，田新朋，李慧贤，张海波*. 中国南海来源海洋链霉菌SCSIO 11863中Enterocins的分离鉴定[J]. 天然产物研究与开发, 2014, 26(8): 1216-1220.

Kumar Saurav, ZHANG Qing-bo, LI Su-mei, ZHANG Wen-jun, TIAN Xin-peng, LI Hui-xian, ZHANG Hai-bo*. Isolation and Characterisation of Enterocins from Marine Streptomyces sp.SCSIO 11863 Isolated from South China Sea[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(8): 1216-1220.

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