冬凌草活性部位逆转SGC7901/ADR 细胞多药耐药性的体外研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (8): 1281-1284.

冬凌草活性部位逆转SGC7901/ADR 细胞多药耐药性的体外研究

高巧慧，许文婷，谢婷，刘瑞媛，吴臻，李发荣*

药用资源与天然药物化学教育部重点实验室，西北濒危药材资源开发国家工程实验室，陕西师范大学生命科学学院，西安 710062

出版日期:2014-08-30 发布日期:2014-11-06

Reversal of Multidrug Resistance in SGC7901/ADR Cells by Rabdosia rubescens

GAO Qiao-hui, XU Wen-ting, XIE Ting, LIU Rui-yuan, WU Zhen, LI Fa-rong*

Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry,Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China,College of Life Sciences,Shaanxi Normal University,Shaanxi Xi’an 710062 ,China

Online:2014-08-30 Published:2014-11-06

摘要/Abstract

摘要： 以SGC7901和SGC7901/ADR为细胞模型，检测了冬凌草活性部位与化疗药物联用后，对SGC7901/ADR耐药性的逆转效应；冬凌草活性部位处理细胞后，检测耐药细胞内阿霉素的蓄积变化、耐药细胞P-糖蛋白（P-gp）的表达水平以及mdr1基因的表达变化。结果显示，冬凌草氯仿部位和乙酸乙酯部位可以有效提高化疗药物阿霉素在SGC7901/ADR细胞内的蓄积，降低P-gp的表达，降低mdr1基因的转录。冬凌草逆转胃癌耐药细胞SGC7901/ADR多药耐药性的活性部位是冬凌草氯仿部位和乙酸乙酯部位，其逆转作用与抑制P-gp的表达相关。

关键词: 冬凌草, SGC7901/ADR, 多药耐药, P-糖蛋白

Abstract: The effect of Rabdosia rubescens on the expression and function of P-glycoprotein (P-gp) in SGC7901/ADR cell was investigated in this study. The potential bioactive extract fractions were selected to carry out experiments in SGC7901/ADR cells. After treatment with chloroform fraction and ethyl acetate fraction of R. rubescens, the cell viability was examined by MTT assay,the expression of P-gp in SGC7901/ADR and SGC7901 cells was determined by fluorescent-labeled antibody technique,and the expression of mdr1 gene was detected by RT-PCR. The experimental results indicated that chloroform fraction and ethyl acetate fraction of R. rubescens increased the intracellular accumulation of adriamycin in SGC7901/ADR cells,and decreased the expression of mdr1 gene and P-gp. Therefore, chloroform fraction and ethyl acetate fraction of R. rubescens had effects on reversal of multidrug resistance of SGC7901/ADR cells,and the mechanism was possibly associated with the inhibition of the expression of P-gp.

Key words: Rabdosia rubescens, SGC7901/ADR, multidrug resistance, P-glycoprotein

中图分类号:

R285.5 Q946

高巧慧，许文婷，谢婷，刘瑞媛，吴臻，李发荣*. 冬凌草活性部位逆转SGC7901/ADR 细胞多药耐药性的体外研究[J]. 天然产物研究与开发, 2014, 26(8): 1281-1284.

GAO Qiao-hui, XU Wen-ting, XIE Ting, LIU Rui-yuan, WU Zhen, LI Fa-rong*. Reversal of Multidrug Resistance in SGC7901/ADR Cells by Rabdosia rubescens[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(8): 1281-1284.

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