王不留行黄酮苷对过氧化氢和高糖诱导 损伤的人脐静脉内皮细胞的保护作用

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (7): 1009-1013.

王不留行黄酮苷对过氧化氢和高糖诱导损伤的人脐静脉内皮细胞的保护作用

谢凤珊1，冯磊2，马丽萍1，邱丽颖1,2*

1 江南大学药学院；2 江南大学无锡医学院，无锡 214122

出版日期:2014-07-31 发布日期:2014-11-09

Protective Effect of Vaccarin on Human EA·hy926 Endothelial Cells Injured by Hydrogen Peroxide and High Glucose

XIE Feng-shan1, FENG Lei2, MA Li-ping1, QIU Li-ying1,2*

1 School of Pharmaceutical Science,Jiangnan University;2 Wuxi Medical School,Jiangnan University,Jiangsu Wuxi 214122 ,China

Online:2014-07-31 Published:2014-11-09

摘要/Abstract

摘要： 体外培养人脐静脉内皮细胞，乳酸脱氢酶（LDH）试剂盒评价王不留行黄酮苷孵育24 h后的细胞毒性。先给予13.76、6.88、3.44 μmol/L的王不留行黄酮苷和维生素C阳性对照组（浓度为100 μmol/L）预孵育12 h后，再分别以H₂O₂和高糖诱导内皮细胞损伤。SRB法测定细胞活力，试剂盒检测乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量。与模型组相比，王不留行黄酮苷13.76、6.88、3.44 μmol/L组均可改善H₂O₂和高糖损伤模型的细胞活力，尤以13.76 μmol/L作用最为显著（P<0.01）；并且王不留行黄酮苷13.76 μmol/L显著降低H₂O₂和高糖损伤组培养液中LDH和MDA 释放量，增强细胞内SOD活性。

关键词: 王不留行黄酮苷, 人脐静脉内皮细胞, H₂O₂损伤, 高糖损伤

Abstract: The cytotoxicity of vaccarin on human EA·hy926 endothelial cells was investigated by measuring the release of lactate dehydrogenase (LDH) in extracellular fluid.The model of oxidative injury was induced by hydrogen peroxide (H₂O₂) and the model of high glucose injury was induced by high glucose after treatment of these cells with different doses (13.76,6.88,3.44 μmol/L) of vaccarin and 100 μmol/L of vitamin C.Then the cell viability was measured by SRB method.The release of LDH,the level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in EA·hy926 were determined according to the manufacturer’s instructions.Hence,the protective effect of vaccarin against H₂O₂ and high glucose induced endothelial cellular damage was confirmed.As shown,the superior effect of vaccarin was observed at 13.76 μmol/L,which significantly reduced the level of MDA and LDH leakage,and enhanced production of SOD.

Key words: vaccarin, human EA·, hy926 endothelial cells, H₂O₂ injury, high glucose injury

中图分类号:

R284.1

谢凤珊1，冯磊2，马丽萍1，邱丽颖1,2*. 王不留行黄酮苷对过氧化氢和高糖诱导损伤的人脐静脉内皮细胞的保护作用[J]. 天然产物研究与开发, 2014, 26(7): 1009-1013.

XIE Feng-shan1, FENG Lei2, MA Li-ping1, QIU Li-ying1,2*. Protective Effect of Vaccarin on Human EA·hy926 Endothelial Cells Injured by Hydrogen Peroxide and High Glucose[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(7): 1009-1013.

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