安子合剂对构建抗心磷脂抗体阳性流产模型小鼠mmp2、mmp9表达的影响

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (6): 967-969.

安子合剂对构建抗心磷脂抗体阳性流产模型小鼠mmp2、mmp9表达的影响

丁超1*，陆启滨2

1 南京中医药大学；2 南京中医药大学附属江苏省中医院,南京210029

出版日期:2014-06-28 发布日期:2014-11-12

Anziheji Effect on mmp2 and mmp9 of ACA Positive Abortion in Mice

DING Chao1*, LU Qi-bin2

1 Nanjing university of Chinese medicine;2 Chinese medicine hospital of jiangsu province,Nanjing 210029 ,China

Online:2014-06-28 Published:2014-11-12

摘要/Abstract

摘要： 从细胞增殖的角度研究安子合剂对ACA阳性流产小鼠母胎界面mmp2、mmp9蛋白的表达及意义。方法：采用Westen Blot方法分别检测mmp2、mmp9在ACA阳性流产模型组、空白对照组、安子合剂低/中/高剂量组和阿司匹林组母胎界面胎盘与蜕膜中蛋白水平的表达。通过Image pool3.0软件分析显示①ACA阳性先兆流产模型小鼠胎盘中mmp2灰度积比值（0.152）较空白对照组（0.611）降低，安子合剂低剂量组mmp2灰度积比值（0.451）接近空白对照组；胎盘中安子合剂低剂量组mmp9灰度积比值（0.497）接近空白对照组（0.644）；②蜕膜中安子合剂低剂量组mmp2灰度积比值（0.692）接近空白对照组（0.232）；蜕膜中安子合剂低剂量组mmp9灰度积比值（1.085）接近空白对照组（1.627）。安子合剂能够调节滋养层细胞的增殖与mmp2、mmp9蛋白表达之间的平衡。

关键词: 安子合剂, 抗心磷脂抗体, 流产, mmp2, mmp9

Abstract: From the perspective of cell proliferation,researching Anziheji on the expression and meaning of mmp2 and mmp9,which are on maternal-fetal interface of the ACA positive threatened abortion in mice. Using Westen blot method to detect the protein levels expression of mmp2 and mmp9 in the placenta and decidua of model group and normal group,Anziheji low/medium/high dose group and aspirin group. The results of the research showed①the Gray scale deposition ratio of mmp2 in the placenta of ACA positive threatened abortion model mice(0.152)is lower than the normal group(0.611),the Gray scale deposition ratio of mmp2 in the placenta of the low dose(0.451)is close to the normal group;the Gray scale deposition ratio of mmp9 in the placenta of the low dose(0.497)is close to the normal group(0644);②the Gray scale deposition ratio of mmp2 in the decidua of the low dose(0.692)is close to the normal group(0.232);the Gray scale deposition ratio of mmp9 in the decidua of the low dose (1.085)is close to the normal group(1.627). Anziheji can regulate the balance of the proliferation in the trophoblast cell and the expression of mmp2,mmp9.

Key words: Anziheji, ACA, abortion, mmp2, mmp9

中图分类号:

R284.2

丁超1*，陆启滨2. 安子合剂对构建抗心磷脂抗体阳性流产模型小鼠mmp2、mmp9表达的影响[J]. 天然产物研究与开发, 2014, 26(6): 967-969.

DING Chao1*, LU Qi-bin2. Anziheji Effect on mmp2 and mmp9 of ACA Positive Abortion in Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(6): 967-969.