天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (3): 450-453.

• 开发研究 • 上一篇    下一篇

树脂法提取纯化白藜芦醇的新工艺研究

徐月灿*,马德新   

  1. 湖南希尔天然药业有限公司,永州 425000
  • 出版日期:2014-03-29 发布日期:2014-12-08

A New Method Developed for the Extraction and Purification of Resveratrol

XU Yue-can*, MA De-xin   

  1. Hill Pharmaceutical Co.,Ltd.,Hunan Yongzhou 425000,China
  • Online:2014-03-29 Published:2014-12-08

摘要: 对虎杖中的白藜芦醇先酶解再提取,提取液采用一种新树脂进行纯化分离。考察了酶解物、不同树脂、上柱量、洗脱量等因素对白藜芦醇提取纯化的影响,确立了白藜芦醇的最佳提取纯化条件:用酿酒曲于35~40 ℃将原药材先酶解24 h,再用75%乙醇提取,提取液经回收乙醇后上XC-7大孔树脂柱,先加10%乙醇洗脱,然后再用60%乙醇洗脱并收集洗脱液,浓缩,真空干燥即可,产品中白藜芦醇含量达85%,白藜芦醇转化率达85%以上。

关键词: 白藜芦醇, 虎杖, 酶解, 大孔树脂

Abstract: In this study,resveratrol was extracted from the fermented giant knotweed.A new resin was applied to purify the extracted solution.The influencing factors of resveratrol extraction and purification results,including enzyme,resin,sample amount used for column chromatography and elution volume were investigated.The optimal conditions were determined to be:enzymolysis of raw material with distiller's yeast for 24 h under 35-40 ℃,extract with 75% ethanol,purify with XC-7 macroporous resin column chromatography,use 10% to 60% ethanol to elute.After concentrating,vacuum drying,the content of resveratrol in product reached 85%.The conversion rate of resveratrol was hence higher than 85%.

Key words: resveratrol, giant knotweed, enzymolysis, macroporous resin

中图分类号: 

R932