关键词: 石榴, HPLC, 山楂酸, 齐墩果酸, 熊果酸, 科罗索酸

Abstract: In this study,a fast and sensitive HPLC method has been developed for the determination of triterpenic acids using 2-(7H-dibenzo［a,g］carbazol-7-yl)ethyl 4methylbenzenesulfonate (DBCETS) as the fluorescent labeling reagent.A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling including labeling time,reaction temperature and the concentration of labeling reagent.Under the optimized conditions,the proposed method gave good reproducibility and accuracy.The linear correlation coefficients for all triterpenic acid derivatives were of >0.9995,and the limit of detections were in the range of 1.10~1.48 ng/mL (S/N=3).The proposed method has been firstly applied for the determination of corosolic acid,maslinic acid,oleanolic acid and ursolic acid in pomegranate,and got satisfactory results.

Key words: pomegranate, HPLC, maslinic acid, oleanolic acid, ursolic acid, corosolic acid