天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 83-86.

• 研究简报 • 上一篇    下一篇

不同产地猫爪草药材中总糖与黄酮含量比较

李自红,范毅,魏悦,郭唯*   

  1. 河南省生物技术开发中心,郑州 450002
  • 出版日期:2014-12-30 发布日期:2015-03-04

Comparison of Total Sugar and Flavone Contents in Radix Ranunculi ternati from Different Places

LI Zi-hong,FAN Yi,WEI Yue,GUO Wei*   

  1. Biotechnology Developing Center,Henan Academy of Sciences,Zhengzhou 450002,China
  • Online:2014-12-30 Published:2015-03-04

摘要: 比较不同产地中猫爪草药材中总糖和黄酮的含量。以葡萄糖为观测指标,采用蒽酮硫酸比色法,测定猫爪草中总糖的含量;以芦丁为观测指标,采用NaNO2-Al(NO3)3-NaOH 为显色体系,利用分光光度法测定其黄酮的含量。河南信阳、安徽、浙江、山西、山东等地猫爪草中多糖含量分别为:18.93%、15.46%、11.78%、9.21%、9.46%,黄酮含量分别为:0.95%、0.32%、0.47%、0.19%、0.28%。不同产地猫爪草中总糖和黄酮含量差异明显,其中以河南信阳猫爪草中总糖和黄酮含量都最高。

关键词: 猫爪草, 总糖, 黄酮, 分光光度法, 含量测定

Abstract: To compare total sugar and flavone contents in Radix Ranunculi Ternati from different places.Anthronevitriol colorimetry was used to determine total sugar content in Radix Ranunculi ternati using glucose as observation index.Spectrophotometry was used to determine flavone contents using rutin as observation index and NaNO2-Al(NO3)3-NaOH as coloration system.The contents of total sugar in Radix Ranunculi Ternati derived from Xinyang Henan,Anhui,Zhejiang,Shanxi and Shandong were 18.93%,15.46%,11.78%,9.21%,9.46%,the contents of flavone were 0.95%,0.32%,0.47%,0.19%,0.28%,respectively.Total sugar and flavone contents in Radix Ranunculi ternati from different places are significantly different,and the highest is the one from Xinyang Henan.

Key words: Radix Ranunculi Ternati, total sugar, flavone, spectrophotometry, content determination

中图分类号: 

R284.2