天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 110-113.

• 开发研究 • 上一篇    下一篇

黍稷麸皮酚酸提取物抗氧化活性研究

牛伟1*,鹿茸2,乔治军3,张丽珍2   

  1. 1山西省农业科学院;2 山西大学生命科学学院;3 农业部黄土高原作物基因资源与种质创制重点实验室 山西省农业科学院农作物品种资源研究所,太原 030006
  • 出版日期:2014-12-30 发布日期:2015-03-04

Antioxidant Activity of Phenolics from Panicum miliaceum L.Bran

NIU Wei1*, LU Rong2, QIAO Zhi-jun3, ZHANG Li-zhen2   

  1. 1 Shanxi Academy of Agricultural Sciences;2 School of Life Science,Shanxi University;3 Key Laboratory aiyuan of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030006,China
  • Online:2014-12-30 Published:2015-03-04

摘要: 以黍稷麸皮Cimi2为研究对象,比较了四种多酚提取方法的效果及提取物清除自由基的能力。从总酚来看,用乙醇浸提法、超声助溶法、酶解法以及超声与酶结合法分别得到225.77、192.23、231.01、221.15 mg没食子酸/100 g样品。四种方法中得到的结合酚和自由酚比例为49:51、29:71,31:69、 28:72。对9个品种的麸皮提取物的体外抗氧化能力检测结果表明,黍稷麸皮酚酸对DPPH、超氧阴离子、羟自由基都有明显的清除作用。其中zigangmi清除DPPH自由基能力和体外总抗氧化能力最强最强,Cimi2的清除羟自由基能力最强。

关键词: 黍稷, 自由酚, 结合酚, 提取方法, 抗氧化能力

Abstract: The bran of proso millet cultivars were analyzed for their phenolic content and antioxidant activities.Four extraction methods,including ethanol solvent、ultrasonicassisted ethanol extraction、enzymolysis、ultrasonicenzymolysis method,were compared for their efficiency of phenolic content of the Cimi2 bran.The total phenolic content were 225.77、192.23、231.01、221.15 mg/100 g respectively.The ratios of bound phenolic to free phenolic were 49:51、29:71、31:69、28:72 for four extraction methods respectively.Total phenolics from proso millet bran has a scavenging activity on DPPH、O2and ·OH radicals.The highest DPPH scavenging ability and total antioxidant capacity was found in zigangmi bran.The highest hydroxyl radical scavenging ability was found in Cimi2 bran.

Key words: Panicum miliaceum L.,free phenolic, bound phenolic, extraction methods, antioxidant activity

中图分类号: 

R284.2