天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl2): 238-241.

• 研究简报 • 上一篇    下一篇

高效液相色谱法测定刺苋中芦丁、槲皮素和山奈酚

秦红英,周光明*,彭贵龙,李俊平,陈军华   

  1. 发光与实时分析教育部重点实验室 西南大学化学化工学院,重庆 400715
  • 出版日期:2014-12-30 发布日期:2015-03-05

Determination of Rutin,Quercetin,Kaempferol in Amaranthus spinosus L. by High Performance Liquid Chromatography

QIN Hong-ying,ZHOU Guang-ming*,PENG Gui-long,LI Jun-ping,CHEN Jun-hua   

  1. Key Laboratory on Luminescence and Real-Time Analysis (Southwest University),Ministry of Education; School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China
  • Online:2014-12-30 Published:2015-03-05

摘要: 本文建立了以甲醇溶液为萃取剂,结合超声辅助萃取,利用高效液相色谱法同时分离测定刺苋中的芦丁、槲皮素和山奈酚三种黄酮的方法。色谱条件为:Phenomenex C18色谱柱(250 mm×4.6 mm,5 μm);流动相为甲醇-0.2%冰醋酸水溶液,并采用梯度洗脱程序;流速 1 mL/min;紫外检测波长360 nm;柱温为35 ℃。结果表明该方法对芦丁、槲皮素和山奈酚均在0.0025~62.5000 μg/mL范围内线性关系良好,相关系数(r)分别为0.99999、0.99997、0.99999;最低检测限(S/N=3)依次为0.1411 ng/mL,0.0795 ng/mL,0.0188 ng/mL,最低定量限(S/N=10)依次为0.4703 ng/mL,0.2652 ng/mL,0.0627 ng/mL;样品回收率为95.79%~101.41%。本法灵敏度高、定量准确且操作简便,为刺苋中芦丁、槲皮素和山奈酚的分离检测提供了有效方法。

关键词: 芦丁, 槲皮素, 山奈酚, 超声辅助萃取, 高效液相色谱法, 刺苋

Abstract: A simple high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of rutin,quercetin,kaempferol in Amaranthus spinosus L.using methanol as extractant with ultrasonic assisted extraction.Chromatographic separation was carried out using a Phenomenex C18 column with a gradient elution,the mobile phase was methanol-0.2% acetic acid solution with the UV detection wavelength set at 360 nm and the flow rate of 1.0 mL/min.The linearity range for rutin,quercetin,kaempferol were found to be 00.002562.5000 μg/mL,and the correlation coefficients were 0.99999, 0.99997, 0.99999, respectively.The limits of detection (LOD,S/N=3) were 0.1411 ng/mL,0.0795 ng/mL,0.0188 ng/mL,respectively.The limit of quantification (LOQ,S/N=10) were 0.4703 ng/mL,0.2652 ng/mL,0.0627 ng/mL,respectively.Accuracy of the method was checked by conducting recovery studies at three different concentration levels for all the three compounds and the average recoveries were ranged from 95.79% to 101.41%.The developed HPLC method was found to be simple,precise,specific,sensitive and accuracy.It provided a new and scientific mean for routine quality control of Amaranthus spinosus L.

Key words: rutin, quercetin, kaempferol, ultrasonic-assisted extraction, high performance liquid chromatography, Amaranthus spinosus L.

中图分类号: 

O652.62